High-resolution, noninvasive longitudinal live imaging of immune responses
High-resolution, noninvasive longitudinal live imaging of immune responses
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DOI：
10.1073/pnas.1105002108
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发表时间：
2011-08-02
影响因子：
11.1
通讯作者：
Berggren, Per-Olof
中科院分区：
文献类型：
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作者：
Abdulreda, Midhat H.;Faleo, Gaetano;Berggren, Per-Olof
活体成像是生物学研究中不可缺少的工具，各种成像技术已经被开发出来，以非侵入性地监测活体组织。然而，目前的大多数技术都缺乏在单细胞水平上研究事件的分辨率。尽管活体内多光子显微镜已经解决了这一局限性，但在纵向体内研究中，重复非侵入性接触相同组织的需求在很大程度上仍未得到满足。我们现在报告一种以前未探索的方法来研究胰岛移植到小鼠眼前房后的免疫反应。这一方法使得(I)体内移植组织的纵向、非侵入性成像；(Ii)体内细胞标记以原位评估细胞表型和生存能力；(Iii)通过局部应用或眼内注射进行局部干预；以及(Iv)实时跟踪目标组织中渗透的免疫细胞。
Intravital imaging emerged as an indispensible tool in biological research, and a variety of imaging techniques have been developed to noninvasively monitor tissues in vivo. However, most of the current techniques lack the resolution to study events at the single-cell level. Although intravital multiphoton microscopy has addressed this limitation, the need for repeated noninvasive access to the same tissue in longitudinal in vivo studies remains largely unmet. We now report on a previously unexplored approach to study immune responses after transplantation of pancreatic islets into the anterior chamber of the mouse eye. This approach enabled (i) longitudinal, noninvasive imaging of transplanted tissues in vivo; (ii) in vivo cytolabeling to assess cellular phenotype and viability in situ; (iii) local intervention by topical application or intraocular injection; and (iv) real-time tracking of infiltrating immune cells in the target tissue.