Measurement of intercellular cohesion by tissue surface tensiometry.
Measurement of intercellular cohesion by tissue surface tensiometry.
复制标题
通过组织表面张力测定法测量细胞间凝聚力。
DOI：
10.1007/978-1-4939-1164-6_16
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Foty,RamseyA
中科院分区：
文献类型：
--
作者：
Foty,RamseyA
细胞间粘附在许多生物学过程中起着至关重要的作用，包括胚胎发育、恶性侵袭和伤口愈合，并且可以在组织工程应用中被操纵以产生复杂的结构。精确测量细胞间粘附的强度并不简单，需要基于合理物理原理的方法。组织表面张力测定法（TST）严格量化了生理条件下3D组织样聚集体的细胞间内聚能。TST利用定制的张力计来压缩平行板之间的3D球体。对所施加的力的阻力和聚集体几何形状的变化被应用于杨-拉普拉斯方程，产生表观表面张力的测量。我们描述了组成张力计的所有组件，并提供了生成球形聚集体所涉及的所有关键步骤的分步说明。我们解释了如何计算组织表面张力，并提供了一个样本数据集从12个聚集的统计分析。
Intercellular adhesion plays a vital role in many biological processes including embryonic development, malignant invasion, and wound healing, and can be manipulated to generate complex structures in tissue engineering applications. Accurate measurement of the strength of intercellular adhesion is not trivial and requires methods rooted in sound physical principles. Tissue surface tensiometry (TST) rigorously quantifies intercellular cohesive energy of 3D tissue-like aggregates under physiological conditions. TST utilizes a custom-built tensiometer to compress 3D spheroids between parallel plates. The resistance to the applied force and changes in aggregate geometry are applied to the Young-Laplace equation, generating a measurement of apparent surface tension. We describe all components comprising the tensiometer and provide step by step instructions of all the key steps involved in generating spherical aggregates. We explain how tissue surface tension is calculated and provide a statistical analysis of a sample data set from 12 aggregates.