Microdialysis sampling and chemiluminescence detection for in vivo and real-time study of the lead metabolism in rabbit blood
Microdialysis sampling and chemiluminescence detection for in vivo and real-time study of the lead metabolism in rabbit blood
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DOI：
10.1007/s00604-006-0699-2
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发表时间：
2007-07
影响因子：
5.7
通讯作者：
X. Hou;Z. Zhang;Yan Zhao;Jie Ma
中科院分区：
文献类型：
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作者：
X. Hou;Z. Zhang;Yan Zhao;Jie Ma
研究了K2MnO4-鲁米诺化学发光体系对铅(II)的测定。该方法基于铅(II)对化学发光反应的催化作用。线性范围为3×10−3~9×10−1 mg L−1(r=0.9971)，相对标准偏差(R.S.D.)对于5×10−2 mg L，−1PB(II)测定为0.7%(n=11)。检出限为3×10−4 mg L−1(3σ)铅(II)。在此基础上，建立了一套在体、在线、实时监测兔血中游离铅代谢的分析系统。将微透析探头植入兔静脉，以5 ·L·min· ·−·1的流量灌流，用流动注射化学发光系统在线测定透析液中的游离铅浓度。该系统包括微渗析采样、在线分离和化学发光检测。铅(II)的浓度-时间曲线符合一室开放模型，消除半衰期(t1/2)为37.77min，峰时(Tmax)为85.20min，峰浓度(Cmax)为0.137 mg−-1。
The chemiluminescence (CL) system K2MnO4-luminol is shown to be useful for the determination of lead(II). The method is based on the catalytic effect of Pb(II) on the CL reaction. The linear range was 3 × 10−3–9 × 10−1mg L−1(r= 0.9971) and the relative standard deviation (R.S.D.) for 5 × 10−2mg L−1Pb(II) measurement was 0.7% (n= 11). The detection limit was 3 × 10−4mg L−1(3σ) Pb(II). Based on this, an in vivo, on-line, real-time analytical system for monitoring the metabolism of free lead(II) in rabbit blood was developed. A microdialysis probe, implanted in the vein of a rabbit, was perfused with perfusate at a flow rate of 5 µL min−1. The concentration of free lead(II) in the dialysate was determined on-line with a flow-injection CL system. This system included microdialysis sampling, on-line separation and chemiluminescence detection. The concentration-time curve of lead(II) was in accordance with the one-compartmental open model, T1/2(elimination half-life), Tmax(peak time) and Cmax(peak concentration) were 37.77 min, 85.20 min and 0.137 mg L−1, respectively.