Prospective identification of myogenic endothelial cells in human skeletal muscle
Prospective identification of myogenic endothelial cells in human skeletal muscle
复制标题
DOI：
10.1038/nbt1334
复制
发表时间：
2007-09-01
影响因子：
46.9
通讯作者：
Peault, Bruno
中科院分区：
文献类型：
--
作者：
Zheng, Bo;Cao, Baohong;Peault, Bruno
我们的文件解剖，分子和人类骨骼肌内的内皮细胞和肌原细胞之间的发育关系。用免疫组化和流式细胞术鉴定共表达肌源性和内皮细胞标志物（CD56，CD34，CD144）的细胞。在严重联合免疫缺陷小鼠的受损骨骼肌中，这些肌内皮细胞比CD56（+）肌源性祖细胞更有效地再生肌纤维。它们能长期增殖，保持正常的核型，不会致瘤，并且在氧化应激下比CD56（+）肌源性细胞存活得更好。克隆来源的肌内皮细胞在培养中分化为成肌细胞、成骨细胞和成软骨细胞。肌内皮细胞适合于生物技术处理，包括通过流式细胞术纯化和体外长期扩增，并且可能具有治疗人类肌肉疾病的潜力。
We document anatomic, molecular and developmental relationships between endothelial and myogenic cells within human skeletal muscle. Cells coexpressing myogenic and endothelial cell markers (CD56, CD34, CD144) were identified by immunohistochemistry and flow cytometry. These myoendothelial cells regenerate myofibers in the injured skeletal muscle of severe combined immunodeficiency mice more effectively than CD56(+) myogenic progenitors. They proliferate long term, retain a normal karyotype, are not tumorigenic and survive better under oxidative stress than CD56(+) myogenic cells. Clonally derived myoendothelial cells differentiate into myogenic, osteogenic and chondrogenic cells in culture. Myoendothelial cells are amenable to biotechnological handling, including purification by flow cytometry and long-term expansion in vitro, and may have potential for the treatment of human muscle disease.