TRANSCRIPTIONAL REPRESSION MEDIATED BY THE WT1 WILMS-TUMOR GENE-PRODUCT
TRANSCRIPTIONAL REPRESSION MEDIATED BY THE WT1 WILMS-TUMOR GENE-PRODUCT
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DOI：
10.1126/science.1654597
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发表时间：
1991-09-27
期刊：
影响因子：
56.9
通讯作者：
RAUSCHER, FJ
中科院分区：
文献类型：
--
作者：
MADDEN, SL;COOK, DM;RAUSCHER, FJ
wt1 基因是一种假定的肿瘤抑制基因，位于染色体 11p13 上的肾母细胞瘤 (WT) 基因座上，编码一种含锌指的蛋白质，该蛋白质与 EGR-1 结合相同的 DNA 序列，EGR-1 是一种激活转录的有丝分裂原诱导的立即早期基因产物。 WT1 的转录调控潜力尚未得到证实。在瞬时转染测定中，WT1 蛋白在与 EGR-1 位点结合时起到转录抑制因子的作用。抑制功能被映射到 WT1 富含谷氨酰胺和脯氨酸的 NH2 末端；该结构域与 EGR-1 锌指区域的融合将 EGR-1 转化为转录抑制因子。
The wt1 gene, a putative tumor suppressor gene located at the Wilms tumor (WT) locus on chromosome 11p13, encodes a zinc finger-containing protein that binds to the same DNA sequence as EGR-1, a mitogen-inducible immediate-early gene product that activates transcription. The transcriptional regulatory potential of WT1 has not been demonstrated. In transient transfection assays, the WT1 protein functioned as a repressor of transcription when bound to the EGR-1 site. The repression function was mapped to the glutamine- and proline-rich NH2-terminus of WT1; fusion of this domain to the zinc finger region of EGR-1 converted EGR-1 into a transcriptional repressor.