The lumicins:: novel bacteriocins from Photorhabdus luminescens with similarity to the uropathogenic-specific protein (USP) from uropathogenic Escherichia coli
The lumicins:: novel bacteriocins from Photorhabdus luminescens with similarity to the uropathogenic-specific protein (USP) from uropathogenic Escherichia coli
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DOI：
10.1111/j.1574-6968.2002.tb11354.x
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发表时间：
2002-09-10
影响因子：
2.1
通讯作者：
ffrench-Constant, R
中科院分区：
文献类型：
--
作者：
Sharma, S;Waterfield, N;ffrench-Constant, R
细菌素是由细菌产生的蛋白质，用于破坏占据其生态位的其他细菌。发光杆菌是一种由昆虫病原线虫携带的昆虫病原细菌，在其生活史中占据几个不同的生态位。线虫进入昆虫体内，释放出一种发光拟单胞菌。然后细菌杀死宿主，细菌和线虫在尸体内复制。引人注目的是，在感染结束时，尸体仍然被单一菌株的细菌所占据，这表明发光假单胞菌可以摧毁进入或存在于昆虫体内的其他细菌。在这里，我们描述了四个基因座编码的'发光蛋白'在P.发光亚种。阿赫斯特氏菌菌株W14。光菌素是一种新型的细菌素，能够杀死发光杆菌属和大肠杆菌的其他菌株。这些位点预测了杀伤蛋白和多种具有类似于绿脓菌素和大肠杆菌素结构域的双重免疫蛋白。杀伤蛋白本质上是具有多个结构域的嵌合体，其中一个结构域类似于由尿路致病性大肠杆菌描述的尿路致病性特异性蛋白（USP）。杆菌这些新的细菌素对光杆状菌的生活方式的影响以及USP作为细菌素在大肠杆菌中的潜在作用。大肠杆菌中进行了讨论。(C)2002年，欧洲微生物学会联合会。由Elsevier Science B.V.出版，版权所有。
Bacteriocins are proteins produced by bacteria to destroy other bacteria occupying their ecological niche. Photorhabdus luminescens is an insect pathogenic bacterium carried by an entomopathogenic nematode and occupies several different niches in its life cycle. The nematode enters the insect and releases a single strain of P. luminescens. The bacteria then kill the host and the bacteria and nematodes replicate within the cadaver. Strikingly, at the end of the infection the cadaver is still occupied by a single strain of bacterium, suggesting that P. luminescens can destroy other bacteria entering, or present within, the insect. Here we describe four loci encoding 'lumicins' in P. luminescens subsp. akhurstii strain W14. The lumicins are novel bacteriocins capable of killing other strains of Photorhabdus and Escherichia coli. These loci predict killer proteins and multiple dual type immunity proteins with domains similar to pyocins and colicins. The killer proteins are chimeric in nature with multiple domains, one of which is similar to the uropathogenic-specific protein (USP) described from uropathogenic E. coli. The implications of these novel bacteriocins for the lifestyle of Photorhabdus and the potential role of USP as a bacteriocin in E. coli are discussed. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.