Comparison of three commercially available antibodies for flow cytometric monitoring of P-glycoprotein expression in tumor cells.
Comparison of three commercially available antibodies for flow cytometric monitoring of P-glycoprotein expression in tumor cells.
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用于流式细胞术监测肿瘤细胞中 P-糖蛋白表达的三种市售抗体的比较。
DOI：
10.1002/cyto.990120807
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Stein,JH
中科院分区：
文献类型：
--
作者：
Krishan,A;Sauerteig,A;Stein,JH
Cellular drug resistance to natural products is often due to the presence of an efflux pump which reduces intracellular drug content and chemosensitivity. A 170 kD cell surface resident P‐glycoprotein is believed to act as the efflux pump. In the present report, we have compared three commercially available antibodies C‐219, JSB‐1, and mdr(Ab‐1) for use in flow cytometric detection of P‐glycoprotein positive cells. Our data show that C219 gives uniformly good results in a variety of murine and human tumor cell lines for detection of P‐glycoprotein positive cells. We have also compared data of C219 stained cells analyzed in parallel on a flow cytometer equipped with a small laser (15 mW) and a large laser (5 watt) cell sorter. Data obtained on these two instruments are comparable. A staining protocol and data on dual staining of cells for DNA content by propidium iodide and P‐glycoprotein expression after FITC labeling are also presented.
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