Characterisation of Pb-resistant plant growth-promoting rhizobacteria (PGPR) from Scirpus grossus
Characterisation of Pb-resistant plant growth-promoting rhizobacteria (PGPR) from Scirpus grossus
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DOI：
10.1016/j.bcab.2019.101456
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发表时间：
2020-01-01
影响因子：
4
通讯作者：
Idris, M.
中科院分区：
文献类型：
--
作者：
Kamaruzzaman, M. A.;Abdullah, S. R. S.;Idris, M.
具有提高植物生产力和健康的潜力的微生物被称为促进植物生长的根际细菌。根际细菌通过提高植物修复效率，在植物修复过程中发挥着重要作用，因此寻找优势根际细菌的必要性日益受到重视。在温室环境下，用塑料板条箱将罗汉果草暴露于不同浓度的铅污染沙子(50、100、150、200和300 mg/L)中。暴露4周后，植株可耐受高达300 mg/L的铅污染。从罗汉果根中分离到22个根际细菌菌落。在细胞和菌落观察的基础上对根际细菌进行了鉴定。通过对16S rDNA序列的分析，对分离到的根际细菌进行了鉴定，鉴定为台湾气单胞菌5E，芽孢杆菌5E。7G、蜡样芽孢杆菌8H和3C、绒毛芽孢杆菌9I、蛋白水解性芽孢杆菌4D、平流层芽孢杆菌14N、巨大芽孢杆菌11K、假单胞菌。菌株12L、阴沟肠杆菌13M和16P、气芽孢杆菌15O和溶杆菌属。分离出10J。经鉴定，耐铅根际细菌分别为蛋白溶解芽孢杆菌4D、维氏芽孢杆菌9I和溶菌素杆菌。10J菌株，能耐受高浓度铅(300 mg/L)。结果表明，这些高度耐铅的根际细菌具有产生植物生长促进性状的潜力，并具有促进罗汉果生长和提高生产力的能力。
Microorganisms that have the potential to improve plant productivity and health are known as plant growth-promoting rhizobacteria. Rhizobacteria play a major role in phytoremediation process by increasing the phytoremediation efficiency, thus the need to identify the superior rhizosphere bacteria has been gaining serious attention. In the current study, Scirpus grossus were exposed to a series of lead-contaminated sand (50, 100, 150, 200 and 300 mg/L) in plastic crates in greenhouse enviroment. Four weeks after exposure, the plants could sustain with toxicity of lead contamination by as much as 300 mg/L. Twenty two colonies of rhizobacteria were isolated from plant roots of Scirpus grossus. The rhizobacteria were characterised based on cell and colony observation. Identification of rhizobacteria was carried out based on analysis of 16S rDNA sequences and were identified as Aeromonas taiwanensis isolate 5E, Bacillus sp. Isolate 7G, Bacillus cereus isolate 8H and 3C, Bacillus velezensis isolate 9I, Bacillus proteolyticus isolate 4D, Bacillus stratosphericus isolate 14N, Bacillus megaterium isolate 11K, Pseudomonas sp. Isolate 12L, Enterobacter cloacae isolate 13M and isolate 16P, Bacillus aerius isolate 15O and Lysinibacillus sp. isolate 10J. The highly lead-resistant rhizobacteria were identified as Bacillus proteolyticus isolate 4D, Bacillus velezensis isolate 9I and Lysinibacillus sp. isolate 10J, which can tolerate against high concentration of lead (300 mg/L). The results showed that these highly lead-resistant rhizobacteria have potential to produce plant growth-promoting traits and have a ability to help S. grossus growth and productivity.