A surface-exposed neuraminidase affects complement resistance and virulence of the oral spirochaete Treponema denticola.
A surface-exposed neuraminidase affects complement resistance and virulence of the oral spirochaete Treponema denticola.
复制标题
DOI：
10.1111/mmi.12311
复制
发表时间：
2013-09
影响因子：
3.6
通讯作者：
Li C
中科院分区：
文献类型：
--
作者：
Kurniyati K;Zhang W;Zhang K;Li C
神经氨酸酶（唾液酸酶）催化从糖缀合物去除末端唾液酸。细菌病原体通常利用神经氨酸酶来吸收宿主唾液酸，唾液酸可以用作营养物或装饰分子来伪装自己免受宿主免疫攻击。在此，一个假定的神经氨酸酶（TDE 0471）被确定在齿垢密螺旋体，口腔螺旋体与人类牙周炎。TDE 0471是一种细胞表面暴露的外切神经氨酸酶，可从人血清蛋白中去除唾液酸;在模拟龈沟液的介质中生长，这表明螺旋体可能使用唾液酸作为体内营养素。TDE 0471保护T.通过防止膜攻击复合物在细菌细胞表面上的沉积而使齿垢免受血清杀伤。动物研究表明，TDE 0471缺陷型突变体在BALB/C小鼠中的毒性低于其亲本野生型菌株。然而，在补体缺陷型B6.129S4-C3 tm 1Crr/J小鼠中，它引起的组织损伤水平与野生型相似，尽管这两种细菌菌株引起的损伤在这些转基因小鼠中更严重。基于这些结果，我们提出T.齿垢菌已经进化出一种策略，利用其神经氨酸酶来吸收宿主唾液酸，这使得螺旋体能够获得营养并逃避补体的杀伤。
Neuraminidases (sialidases) catalyze the removal of terminal sialic acid from glycoconjugates. Bacterial pathogens often utilize neuraminidases to scavenge host sialic acid, which can be utilized either as a nutrient or as a decorating molecule to disguise themselves from host immune attacks. Herein, a putative neuraminidase (TDE0471) was identified in Treponema denticola, an oral spirochete associated with human periodontitis. TDE0471 is a cell surface-exposed exo-neuraminidase that removes sialic acid from human serum proteins; it is required for T. denticola to grow in a medium that mimics gingival crevice fluid, suggesting that the spirochete may use sialic acid as a nutrient in vivo. TDE0471 protects T. denticola from serum killing by preventing the deposition of membrane attack complexes on the bacterial cell surface. Animal studies revealed that a TDE0471-deficient mutant is less virulent than its parental wild-type strain in BALB/C mice. However, it causes a level of tissue damage similar to the wild type in complement-deficient B6.129S4-C3tm1Crr/J mice albeit the damage caused by both bacterial strains is more severe in these transgenic mice. Based on these results, we propose that T. denticola has evolved a strategy to scavenge host sialic acid using its neuraminidase, which allows the spirochete to acquire nutrients and evade complement killing.
登录
查看更多内容
影响因子：
3.2
作者：
Bian, Jiang;Shen, Hongwu;Li, Chunhao
通讯作者：
Li, Chunhao
影响因子：
3.1
作者：
Brooks, CS;Vuppala, SR;Akins, DR
通讯作者：
Akins, DR
影响因子：
8
作者：
Copley, RR;Russell, RB;Ponting, CP
通讯作者：
Ponting, CP
影响因子：
3.5
作者：
BICKEL, M;CIMASONI, G
通讯作者：
CIMASONI, G
影响因子：
3.6
作者：
DRAMSI, S;KOCKS, C;COSSART, P
通讯作者：
COSSART, P