Efficient, trans-complementing packaging systems for chimeric, pseudoinfectious dengue 2/yellow fever viruses.
Efficient, trans-complementing packaging systems for chimeric, pseudoinfectious dengue 2/yellow fever viruses.
复制标题
DOI：
10.1016/j.virol.2009.12.015
复制
发表时间：
2010-04-25
期刊：
影响因子：
3.7
通讯作者：
Frolov I
中科院分区：
文献类型：
--
作者：
Shustov AV;Frolov I
In our previous studies, we have stated to build a new strategy for developing defective, pseudoinfectious flaviviruses (PIVs) and applying them as a new type of vaccine candidates. PIVs combined the efficiency of live vaccines with the safety of inactivated or subunit vaccines. The results of the present work demonstrate further development of chimeric PIVs encoding dengue virus 2 (DEN2V) glycoproteins and yellow fever virus (YFV)-derived replicative machinery as potential vaccine candidates. The newly designed PIVs have synergistically functioning mutations in the prM and NS2A proteins, which abolish processing of the latter proteins and make the defective viruses capable of producing either only noninfectious, immature and/or subviral DEN2V particles. The PIV genomes can be packaged to high titers into infectious virions in vitro using the NS1-deficient YFV helper RNAs, and both PIVs and helpers can then be passaged as two-component genome viruses at an escalating scale.
登录
查看更多内容
影响因子：
9.2
作者：
Haas, J;Park, EC;Seed, B
通讯作者：
Seed, B
影响因子：
5.4
作者：
Huang, CYH;Butrapet, S;Kinney, RM
通讯作者：
Kinney, RM
影响因子：
5.4
作者：
Aberle, JH;Aberle, SW;Mandl, CW
通讯作者：
Mandl, CW
影响因子：
5.4
作者：
Lindenbach, BD;Rice, CM
通讯作者：
Rice, CM
影响因子：
3.7
作者：
Mason, Peter W.;Shustov, Alexandr V.;Frolov, Ilya
通讯作者：
Frolov, Ilya