Genotoxicity evaluation in workers occupationally exposed to lead
Genotoxicity evaluation in workers occupationally exposed to lead
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DOI：
10.1016/j.ijheh.2010.01.005
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发表时间：
2010-03-01
影响因子：
6
通讯作者：
Rahman, M. F.
中科院分区：
文献类型：
--
作者：
Grover, Paramjit;Rekhadevi, P. V.;Rahman, M. F.
铅（Pb）是一种广泛使用的重金属，具有广泛的工业用途。然而，铅是一个严重的公共卫生问题，因为它是最广泛的环境和工业毒素之一。本研究的目的是通过Cornet试验、微核（MN）和染色体畸变（CA）试验来评价铅的遗传毒性。测定血铅、尿铅、红细胞δ-氨基乙酰丙酸脱氢酶（E-ALAD）和尿h-氨基乙酰丙酸（U-ALA）水平。暴露相关的氧化应激也进行了研究。研究组包括90名男性铅回收单位工人和90名匹配的对照组。结果表明，接触组彗星尾长显著高于对照组（P < 0.05）。颊粘膜上皮细胞（BEC）和外周血淋巴细胞（PBL）微核分析显示，有一个显着增加的MN频率在接触者比对照组。异常中期分裂相的频率也被发现显着升高，在铅接触工人。接触者抗氧化酶水平相对降低（P > 0.05），脂质过氧化反应发生率增高。血铅和尿铅浓度高于对照组。与对照组相比，暴露受试者的E-ALAD水平降低，U-ALA水平升高。考虑混杂因素的分析结果为铅暴露与遗传毒性的关系提供了证据，并预测了铅暴露工人患癌症的风险增加。鉴于观察到的结果，可以有力地得出结论，工人属于风险群体，适当的安全、预防和防范措施只能最大限度地减少接触和相关的健康危害。(C)2010年Elsevier GmbH。All rights reserved.
Lead (Pb) is a widely used heavy metal with a broad industrial usage. Nevertheless, Pb is a serious public health issue as it is one of the most widespread environmental and industrial toxins. The aim of this investigation was to assess the genotoxicity of Pb using the cornet assay, micronucleus (MN) and chromosomal aberrations (CA) test. Blood and urinary Pb content, levels of delta-aminolevulinic acid dehydratase in the erythrocytes (E-ALAD) and h-aminolevulinic acid in urine (U-ALA) were determined. The exposure associated oxidative stress was also studied. The study group comprised of 90 male Pb recovery unit workers and 90 matched controls. The results indicated that the exposed workers had a significantly higher mean comet tail length than that of controls (P < 0.05). Analysis of micronuclei in buccal epithelial cells (BEC's) and peripheral blood lymphocytes (PBL) revealed that there was a significant increase in frequency of MN in exposed subjects than controls. The frequency of aberrant metaphases was also found to be significantly elevated in the Pb exposed workers. The levels of antioxidant enzymes were relatively reduced (P > 0.05) while the rate of lipid peroxidation was higher in the exposed subjects. Blood and urinary Pb concentrations were found to be higher in exposed workers than in controls. E-ALAD levels were reduced and U-ALA levels were elevated in the exposed subjects in comparison to controls. Results of analysis, taking the confounding factors into consideration provide evidence for the association of Pb exposure and genotoxicity, and predict the increased risk of cancer to the exposed workers. In view of the observed results, it can be strongly concluded that the workers comprise the risk group and adequate safety, precautionary and preventive measures could only minimize exposure and the related health hazards. (C) 2010 Elsevier GmbH. All rights reserved.