In vivo and in vitro impairment of human and ram sperm nuclear chromatin integrity by sexually transmitted Ureaplasma urealyticum infection
In vivo and in vitro impairment of human and ram sperm nuclear chromatin integrity by sexually transmitted Ureaplasma urealyticum infection
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DOI：
10.1095/biolreprod63.4.1041
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发表时间：
2000-10-01
影响因子：
3.6
通讯作者：
Bartoov, B
中科院分区：
文献类型：
--
作者：
Reichart, M;Kahane, I;Bartoov, B
不育男性精液中解脲脲原体感染的发生率是可变的（7%-42%）。通过常规精液分析积累的证据表明，这种感染可能导致胚胎丢失，而不一定影响精子质量。本研究的目的是专门研究U。解脲支原体感染对精子染色质稳定性和DNA完整性的影响，已知这与妊娠结局相关。从体内感染U.通过核染色质去凝聚试验测定，溶脲支原体显示低百分比的稳定染色质（42% +/-4.8%，n = 8），通过精子染色质结构试验测定，显示高百分比的变性DNA（60.9% +/-9.1%，n = 7）。多西环素治疗后，观察到两个参数的显著改善（分别为73.7% +/-3.6%，P < 0.001和30.1% +/-3.5%，P < 0.008）。体外感染的精子细胞比未感染的细胞表现出更高的存活率和运动率。相比之下，U.溶脲菌引起显著的剂量和时间依赖性染色质去浓缩和DNA损伤。与未感染的对照细胞相比，在100个脲原体/精子的感染复数下，用血清型8和3感染30分钟后，具有变性DNA的人精子细胞的百分比分别显著增加了54.9% +/- 23.9%和47.9% +/-12.1%。DNA损伤在感染的公羊精子中显著更明显（180.9% +/- 21.5%）。这些结果表明，保存精子活动后U，解脲支原体感染导致父亲的DNA损伤，虽然保持了高受精率，胚胎发育可能会因此受损。
The incidence of Ureaplasma urealyticum infection in the semen of infertile men is variable (7%-42%). Evidence has accumulated through routine semen analysis to suggest that this infection can cause embryo loss without necessarily affecting sperm quality. The aim of this study was to specifically investigate the effects of U. urealyticum infection on sperm chromatin stability and DNA integrity, which are known to be correlated to pregnancy outcome. Sperm cells isolated from human semen infected in vivo with U. urealyticum exhibited a low percentage of stable chromatin as determined by nuclear chromatin decondensation assay (42% +/- 4.8%, n = 8) and a high percent of denatured DNA as determined by sperm chromatin structure assay (60.9% +/- 9.1%, n = 7). After doxycyclin treatment, a significant improvement in both parameters was observed (73.7% +/- 3.6%, P < 0.001 and 30.1% +/- 3.5%, P < 0.008, respectively). Sperm cells infected in vitro exhibited higher rates of viability and motility than uninfected cells. In contradistinction, U. urealyticum caused significant dose- and time-dependent chromatin decondensation and DNA damage. The percentage of human sperm cells with denatured DNA increased significantly by 54.9% +/- 23.9% and 47.9% +/- 12.1%, after 30 min infection with serotypes 8 and 3, respectively, at a multiplicity of infection of 100 ureaplasmas per sperm compared with uninfected control cells. The damage to DNA was significantly more pronounced in infected ram sperm (180.9% +/- 21.5%). These results indicate that preserved sperm activity post U, urealyticum infection resulted in damage to paternal DNA, although a high fertilization rate was maintained, and embryonic development may, therefore, be impaired.