A carbon dot based theranostic platform for dual-modal imaging and free radical scavenging
A carbon dot based theranostic platform for dual-modal imaging and free radical scavenging
复制标题
DOI：
10.1039/c9nr05746c
复制
发表时间：
2019-11-21
期刊：
影响因子：
6.7
通讯作者：
Chang, Jia-Yaw
中科院分区：
文献类型：
--
作者：
Lin, Jin-Sheng;Tsai, Yi-Wen;Chang, Jia-Yaw
采用微波热解法在水溶液中合成了掺杂P3+和Mn2+的磁荧光碳点（简称PMn@Cdots）。在该体系中，引入P3+掺杂剂提高了Cdots的发射效率，而Mn2+掺杂剂的存在则提高了Cdots的磁共振成像（MRI）能力。据我们所知，目前的工作是第一次尝试调节PMn@Cdots的红色发射和自由基清除，作为双峰成像纳米探针和抗氧化剂。与大多数发红光的碳点不同，制备的PMn@Cdots可以通过反溶剂沉淀法从未反应的前体中轻松纯化，而不是通过耗时的纯化方法。整个合成过程快速、简便、可高效重现、可扩展。更重要的是，通过流式细胞术、荧光和MRI研究，PMn@Cdots与透明质酸（称为PMn@Cdots/HA）的进一步偶联使它们具有良好的体内和体外生物相容性，以及选择性靶向过表达cd44的癌细胞的能力。同时，PMn@Cdots对多种DPPH、羟基和超氧自由基具有抗氧化活性，其抗氧化活性与抗坏血酸相当。在B16F1、HeLa和HEL细胞中，PMn@Cdots/HA对h2o2诱导的氧化应激具有剂量依赖性的细胞保护能力。因此，基于Cdot的治疗平台可以同时作为潜在的候选治疗方法和双峰探针，在未来的临床应用中实现准确的诊断。
Magnetofluorescent carbon dots (Cdots) doped with both P3+ and Mn2+ (abbreviated as PMn@Cdots) have been synthesized in an aqueous solution via a microwave-assisted pyrolysis method. In this system, a P3+ dopant was introduced to enhance the emission efficiency of the Cdots, while the presence of a Mn2+ dopant granted magnetic resonance imaging (MRI) capability. To the best of our knowledge, the present work is the first attempt to regulate red-emission and free radical scavenging of PMn@Cdots to serve as a dual-modal imaging nanoprobe and an antioxidant agent. Unlike most red-emitting Cdots, the as-prepared PMn@Cdots can be readily purified from unreacted precursors through antisolvent precipitation instead of by time-consuming purification methods. The whole synthetic procedure is rapid, facile, efficiently reproducible, and scalable. More importantly, further conjugation of the PMn@Cdots with hyaluronic acid (termed PMn@Cdots/HA) gives them good in vivo and in vitro biocompatibility as well as the capability to selectively target CD44-overexpressing cancer cells, as investigated by flow cytometry, fluorescence, and MRI. Meanwhile, PMn@Cdots exhibit antioxidant activity against multiple DPPH, hydroxyl, and superoxide radicals, which is comparable to that for ascorbic acid. Favorably, PMn@Cdots/HA showed a dose-dependent cytoprotective capability against H2O2-induced oxidative stress in B16F1, HeLa, and HEL cells. Therefore, the Cdot based theranostic platform can simultaneously function as a potential therapeutic candidate and as a dual-modal probe for enabling accurate diagnosis in future clinical applications.