NMR structure of the mature dimer initiation complex of HIV-1 genomic RNA.
NMR structure of the mature dimer initiation complex of HIV-1 genomic RNA.
复制标题
HIV-1 基因组 RNA 成熟二聚体起始复合物的 NMR 结构。
DOI：
10.1016/s0014-5793(99)01183-7
复制
发表时间：
1999
期刊：
影响因子：
3.5
通讯作者：
James,TL
中科院分区：
文献类型：
--
作者：
Mujeeb,A;Parslow,TG;Zarrinpar,A;Das,C;James,TL
The two identical genomic RNA strands inside each HIV‐1 viral particle are linked through homodimerization of an RNA stem‐loop, termed SL1, near their 5′ ends. SL1 first dimerizes through a palindromic sequence in its loop, forming a transient kissing‐loop complex which then refolds to a mature, linear duplex. We previously reported the NMR structure of a 23‐base truncate of SL1 in kissing‐dimer form, and here report the high‐resolution structure of its linear isoform. This structure comprises three short duplex regions – derived from the central palindrome and two stem regions of each strand, respectively – separated by two bulges that each encompass three unpaired adenines flanking the palindromes. The stacking pattern of these adenines differs from that seen in the kissing‐loop complex, and leads to greater colinear base stacking overall. Moreover, the mechanical distortion of the palindrome helix is reduced, and base pairs ruptured during formation of the kissing‐loop complex are re‐established, so that all potential Watson‐Crick pairs are intact. These features together likely account for the greater thermodynamic stability of the mature dimer as compared to its kissing‐loop precursor.
