Zebrafish gcm2 is required for gill filament budding from pharyngeal ectoderm
Zebrafish gcm2 is required for gill filament budding from pharyngeal ectoderm
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DOI：
10.1016/j.ydbio.2004.09.018
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发表时间：
2004-12-15
影响因子：
2.7
通讯作者：
Lieschke, GJ
中科院分区：
文献类型：
--
作者：
Hogan, BM;Hunter, MP;Lieschke, GJ
咽弓产生了对不同过程至关重要的多个器官，包括胸腺、甲状腺和甲状旁腺。在哺乳动物和斑马鱼之间，胸腺和甲状腺器官发生的几个分子调节器是非常保守的。然而，陆地动物有甲状旁腺，而鱼类有鳃。小鼠转录因子胶质细胞缺失2(Gcm2)在第三咽囊内皮细胞的甲状旁腺原基中特异表达，在小鼠和人类中都是甲状旁腺正常发育所必需的。关于鱼鳃器官发生的分子调控尚不清楚。我们报道了gcm2在斑马鱼咽上皮中的表达，以及表达ge2所需的HOx第3组类似物。值得注意的是，斑马鱼的Gem2表达在咽部上皮的外胚层部分，是鳃丝芽发育所必需的，鳃丝芽是鱼类特有的鳃细丝的前体。这项研究确认了GCM基因在胚胎发育中的另一个作用，并指出了gcm2在不同咽部形态演变中的作用。(C)2004 Elsevier Inc.保留所有权利。
The pharyngeal arches give rise to multiple organs critical for diverse processes, including the thymus, thyroid and parathyroids. Several molecular regulators of thymus and thyroid organogenesis are strikingly conserved between mammals and zebrafish. However, land animals have parathyroids whereas fish have gills. The murine transcription factor Glial cells missing 2 (Gcm2) is expressed specifically in the parathyroid primordium in the endodermal epithelium of the third pharyngeal pouch, and in both mice and humans is required for normal development of parathyroid glands. The molecular regulation of fish gill organogenesis remains to be described. We report the expression of gcm2 in the zebrafish pharyngeal epithelium and a requirement for Hox group 3 paralogs for gem2 expression. Strikingly, zebrafish gem2 is expressed in the ectodermal portion of the pharyngeal epithelium and is required for the development of the gill filament buds, precursors of fish-specific gill filaments. This study identifies yet another role for a GCM gene in embryonic development and indicates a role for gcm2 during the evolution of divergent pharyngeal morphologies. (C) 2004 Elsevier Inc. All rights reserved.