SPECTROSCOPIC STUDIES ON CONFORMATION OF GRAMICIDIN A' - PROTON MAGNETIC-RESONANCE ASSIGNMENTS, COUPLING-CONSTANTS, AND H-D EXCHANGE
SPECTROSCOPIC STUDIES ON CONFORMATION OF GRAMICIDIN A' - PROTON MAGNETIC-RESONANCE ASSIGNMENTS, COUPLING-CONSTANTS, AND H-D EXCHANGE
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DOI：
10.1021/bi00754a001
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发表时间：
1972-01-01
期刊：
影响因子：
2.9
通讯作者：
SETTINE, JM
中科院分区：
文献类型：
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作者：
GLICKSON, JD;MAYERS, DF;SETTINE, JM
J. D. Glickson，D. F. Mayer，J. M. Settine和D. W. Urryf摘要：短杆菌肽的商业制剂（Nutritional Biochemicals）（在本文中称为短杆菌肽A '（GA'））的质子磁共振分析表明，该制剂含有短杆菌肽A（GA）、短杆菌肽B（GB）和短杆菌肽C（GC），比例分别为72：9：19。这些短杆菌肽的不同之处仅在于11位的氨基酸。分析是基于色氨酸吲哚CH共振，苯丙氨酸苯基CH共振（GB）和酪氨酸邻CH共振（GC）的相对强度。GA'在六氘代二甲基亚砜（Me_2SO-tif_6）中的220-MHz质子磁共振谱中的共振被指定为特定的氢。有序构象由以下证据表明，所有这些都与ttl一致。d螺旋先前提出用于跨膜通道中GA'的结构（Urry，D. W.等（1971），Proc. Sci. US 68，672; Urry，D. W.，古德尔，M. C.的方法，Glickson，J.D.，和Mayers，D. F.（1971 a），Proc. Sci. US 68，1907）。(1)从化学上等价的侧链质子观察到不同的共振，除了序列posi-
J. D. Glickson, D. F. Mayers, J. M. Settine, and D. W. Urryf abstract: Analysis of proton magnetic resonances of a commercial preparation (Nutritional Biochemicals) of gramicidin, referred to herein as gramicidin A'(GA'), shows the preparation to contain gramicidin A (GA), gramicidin B (GB), and gramicidin C (GC) in ratios of 72: 9: 19, respec-tively. These gramicidins differ only in the amino acid at position 11. The analysis isbased on the relative intensities of tryptophan indole CH resonances, the phenylalanine phenyl CH resonance(of GB), and the tyrosine ortho CH resonance(of GC). Resonances in the 220-MHz proton magnetic resonance spectrum of GA'in hexadeuteriodimethyl sulfoxide (Me2SO-tif6) are assigned to specific hydrogens. An ordered conformation is indicated by the following evidence, all of which is consistent with the ttl. d helices previously proposed for the structure of GA'in transmembranechannels (Urry, D. W.(1971), Proc. Nat. Acad. Sci. US 68, 672; Urry, D. W., Goodall, M. C., Glickson, J. D., and Mayers, D. F.(1971a), Proc. Nat. Acad. Sci. US 68, 1907).(1) Observation of distinct resonances from side-chain protons that are chemically equivalent except for the sequence posi-