Immunostimulatory in Vitro and in Vivo Effects of a Water-Soluble Extract from Kale
Immunostimulatory in Vitro and in Vivo Effects of a Water-Soluble Extract from Kale
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DOI：
10.1271/bbb.100490
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发表时间：
2011-01-01
影响因子：
1.6
通讯作者：
Sugahara, Takuya
中科院分区：
文献类型：
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作者：
Nishi, Kosuke;Kondo, Ai;Sugahara, Takuya
甘蓝(Brassica oleracea L.var.)Acephala DC.)在人杂交瘤HB4C5细胞和人外周血淋巴细胞中具有刺激免疫球蛋白(Ig)产生的活性。羽衣甘蓝主要活性物质的生化和物理性质为一种相对分子质量大于50 kDa的耐热蛋白质。免疫球蛋白产生刺激因子被认为作用于免疫球蛋白的翻译和/或分泌过程。甘蓝提取物对小鼠肠系膜淋巴结和Peyer‘s小结淋巴细胞也有刺激免疫球蛋白生成的作用。对部分纯化的甘蓝提取物进行LC-ESI-MS/MS分析，结果表明1，5-二磷酸核酮糖-1，5-二磷酸羧化酶/加氧酶(Rubisco)为活性物质。菠菜中的Rubisco确实具有刺激HB4C5细胞产生Ig的活性。这些发现提供了甘蓝作为一种促进健康的食物的另一个有益方面。
The water-soluble fraction of kale (Brassica oleracea L. var. acephala DC.) had immunoglobulin (Ig) production stimulating activity in human hybridoma HB4C5 cells and human peripheral blood lymphocytes. The biochemical and physical properties of the main active substance in kale were found to be a heat-stable protein with a molecular weight higher than 50 kDa. The Ig production-stimulating factors were assumed to act on the translational and/or secreting processes of Igs. This Ig production-stimulating effect was also observed in lymphocytes from the mesenteric lymph node and Peyer's patches of mice that had been administered with the kale extract for 14 d. The partially purified kale extract was analyzed by LC-ESI-MS/MS, the result indicating ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) as an active substance. Rubisco from spinach indeed exhibited Ig production-stimulating activity in HB4C5 cells. These findings provide another beneficial aspect of kale as a health-promoting foodstuff.