Identification of a Novel Streptomyces chattanoogensis L10 and Enhancing Its Natamycin Production by Overexpressing Positive Regulator ScnRII
Identification of a Novel Streptomyces chattanoogensis L10 and Enhancing Its Natamycin Production by Overexpressing Positive Regulator ScnRII
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DOI：
10.1007/s12275-009-0014-0
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发表时间：
2009-08-01
影响因子：
3
通讯作者：
Li, Yong-Quan
中科院分区：
文献类型：
--
作者：
Du, Yi-Ling;Chen, Shi-Fei;Li, Yong-Quan
从中国浙江省的土壤样品中分离出一种能够产生那他霉素的新型链霉菌菌株 1,10。基于rpoB基因和16S rDNA序列的系统发育分析以及表型比较，菌株L10 (CGMCC 2644)被认为是S. chattanoogensis的一个先前未鉴定的菌株。通过筛选菌株L10的粘粒文库和引物步移，获得了scnRI的部分序列和scnRII的全序列，它们是纳他霉素生物合成途径特异性正调控基因的直系同源物。通过将额外的 scnRII 拷贝插入菌株 L10 的染色体中而产生的工程化 S. chattanoogensis D1，其游霉素产量在 YSG 培养基中增加了 3.3 倍，在不含蔗糖的 YEME 培养基中增加了 4.6 倍。
A novel Streptomyces strain, 1,10, which is capable of producing natamycin, was isolated from a soil sample collected from Zhejiang province, China. On the basis of phylogenetic analysis of rpoB gene and 16S rDNA sequences, as well as phenotypic comparison, strain L10 (CGMCC 2644) is proposed to be a previously uncharacterized strain of S. chattanoogensis. By screening a cosmid library of strain L10 and primer walking, a partial sequence of scnRI and the entire sequence of scnRII were obtained, which are orthologues to the pathway-specific positive regulator genes of natamycin biosynthesis in S. natalensis. The engineered S. chattanoogensis D1, generated by inserting an additional copy of scnRII into the chromosome of strain L10, increased its natamycin production by 3.3 fold in YSG medium and 4.6 fold in YEME medium without sucrose.