LOCATION OF STRUCTURAL PROTEINS IN PARTICLES OF RICE GALL DWARF VIRUS
LOCATION OF STRUCTURAL PROTEINS IN PARTICLES OF RICE GALL DWARF VIRUS
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DOI：
10.1099/0022-1317-66-4-811
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发表时间：
1985-01-01
影响因子：
3.8
通讯作者：
SAITO, Y
中科院分区：
文献类型：
--
作者：
OMURA, T;MINOBE, Y;SAITO, Y
用聚丙烯酰胺凝胶电泳法将水稻瘤矮病毒颗粒的蛋白质分为7个组分。估计的MW（×103)分别为183、165、150、143、120、56和45千道尔顿（K）。通过用CsCl处理完整颗粒制备的核心含有183 K、165 K、120 K和56 K蛋白; CsCl平衡离心后从梯度的顶层回收150 K、143 K和45 K蛋白。抗完整颗粒的抗血清主要与150 K和45 K蛋白反应，抗核心颗粒的抗血清主要与183 K和120 K蛋白反应。结果表明，150 K和45 K蛋白位于外衣壳表面，183 K和120 K蛋白位于核心表面，165 K和56 K蛋白位于核心内部，143 K蛋白位于外衣壳内部或核心内部。
Protein from particles of rice gall dwarf virus was resolved into 7 components by polyacrylamide gel electrophoresis. The estimated MW (.times. 103) were 183, 165, 150, 143, 120, 56 and 45 kilodaltons (K). Cores prepared by treating intact particles with CsCl contained the 183K, 165K, 120K, and 56K proteins; the 150K, 143K and 45K proteins were recovered from the top layer of gradients after CsCl equilibrium centrifugation. Antiserum against intact particles reacted mainly with 150K and 45K proteins and antiserum against core particles reacted mainly with 183K and 120K proteins. The results suggest that the 150K and 45K proteins are located on the surface of the outer capsid, that the 183K and 120K proteins are on the surface of the core, that the 165K and 56K proteins are inside the core, and that the 143K protein is either inside the outer capsid or inside the core.