Soehngenia saccharolytica gen. nov., sp nov and Clostridium amygdalinum sp nov., two novel anaerobic, benzaldehyde-converting bacteria
Soehngenia saccharolytica gen. nov., sp nov and Clostridium amygdalinum sp nov., two novel anaerobic, benzaldehyde-converting bacteria
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DOI：
10.1099/ijs.0.02668-0
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发表时间：
2003-11-01
影响因子：
2.8
通讯作者：
Stams, AJM
中科院分区：
文献类型：
--
作者：
Parshina, SN;Kleerebezem, R;Stams, AJM
从处理马铃薯淀粉废水的厌氧上流式厌氧污泥床（UASB）反应器中分离到两株苯偶姻厌氧降解菌。菌株BOR-Y-T将苯甲醛转化为约等摩尔浓度的苯甲酸酯和苄醇。苯甲醛转化不支持生长。菌株BOR-Y-T为革兰氏阳性菌，杆状，细胞中部略增厚。该菌株是一种中温产孢菌，在15至40 ℃之间生长，最佳生长温度为30-37 ℃。最适生长pH为pH 7。0.菌株BOR-Y-T在广泛的碳水化合物和一些其他碳源（包括酵母提取物、半胱氨酸和丝氨酸）上生长。其DNA的G + C含量为42mol%。根据生理特征和16 S rRNA基因序列分析，通过与其系统发育邻居的DNA-DNA杂交证实，菌株BOR-Y-T属于梭菌属的第XII簇的新属，即Soehngenia;建议将模式种命名为Soehngenia saccharolytica（模式菌株BOR-Y-T = DSM 12858（T）ATCC BAA-502（T））。菌株BR-10（T）将苯甲醛还原为苯甲醇。这种转变与增长相结合。在含有酵母提取物的培养基中，苯甲醛的存在导致超过两倍的细胞积累。菌株BR-10（T）是一株革兰氏阳性菌，其特征是细胞呈椭圆形或杆状，两端呈椭圆形，单个、成对或有时成链出现。该菌株是中度嗜热的，在20至60 ℃之间生长，最佳生长温度为45 ℃。最适生长pH为7（.）0和7（.）5.菌株BR-10（T）在包括碳水化合物、酵母提取物、酪蛋白和一些氨基酸的广泛碳源上生长。其DNA的G + C含量为32mol%。如通过16 S rRNA基因序列分析所确定的，菌株BR-10（T）代表梭菌属XIVa簇的一个新种;为该新种提出了名称Clostridium amygdalinum（模式菌株BR-10（T）= DSM 12857（T）= ATCC BAA-501（T））。
Two anaerobic, benzaldehyde-converting bacteria were isolated from an anaerobic upflow anaerobic sludge bed (UASB)-reactor treating potato starch waste water. Strain BOR-Y-T converted benzaldehyde to benzoate and benzylalcohol in approximately equimolar concentrations. Benzaldehyde conversion did not support growth. Strain BOR-Y-T was Gram-positive and rod-shaped, and its cells were slightly thickened in the middle. The strain was a mesophilic spore-former that grew between 15 and 40 degreesC, with optimum growth at 30-37 degreesC. The optimum pH for growth was pH 7(.)0. Strain BOR-Y-T grew on a wide range of carbohydrates and some other carbon sources including yeast extract, cysteine and serine. The G + C content of its DNA was 42 mol%. According to physiological characteristics and 16S rRNA gene sequence analysis, confirmed by DNA-DNA hybridization with its phylogenetic neighbours, strain BOR-Y-T belongs to a novel genus of cluster XII of the clostridia, namely Soehngenia; the name Soehngenia saccharolytica is proposed for the type species (type strain BOR-Y-T = DSM 12858(T) ATCC BAA-502(T)). Strain BR-10(T) reduced benzaldehyde to benzylalcohol. This conversion was coupled to growth. In a medium containing yeast extract, the presence of benzaldehyde resulted in the accumulation of more than twofold more cells. Strain BR-10(T) was a Gram-positive organism that was characterized by oval- or rod-shaped cells with oval ends, which occurred singly, in pairs or sometimes in chains. The strain was moderately thermophilic and grew between 20 and 60 degreesC, with optimum growth at 45 degreesC. The optimum pH for growth was between pH 7(.)0 and 7(.)5. Strain BR-10(T) grew on a wide range of carbon sources including carbohydrates, yeast extract, casein and some amino acids. The G + C content of its DNA was 32 mol%. As determined by 16S rRNA gene sequence analysis, strain BR-10(T) represents a novel species of cluster XIVa of the clostridia; the name Clostridium amygdalinum is proposed for this novel species (type strain BR-10(T) = DSM 12857(T) = ATCC BAA-501(T)).