The BIG protein distinguishes the process of CO(2) -induced stomatal closure from the inhibition of stomatal opening by CO(2).
The BIG protein distinguishes the process of CO(2) -induced stomatal closure from the inhibition of stomatal opening by CO(2).
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BIG 蛋白区分 CO2 诱导的气孔关闭过程和 CO2 抑制气孔打开的过程
DOI：
10.1111/nph.14957
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发表时间：
2018-04
期刊：
影响因子：
--
通讯作者：
Hetherington AM
中科院分区：
文献类型：
--
作者：
He J;Zhang RX;Peng K;Tagliavia C;Li S;Xue S;Liu A;Hu H;Zhang J;Hubbard KE;Held K;McAinsh MR;Gray JE;Kudla J;Schroeder JI;Liang YK;Hetherington AM
我们进行了一个基于红外热成像的遗传筛选，以确定拟南芥突变体显示异常气孔行为响应浓度升高的CO2。这种方法导致分离的一个新的等位基因的拟南芥BIG基因座（At3g02260），我们称之为CO2不敏感1（顺式1）。 BIG突变体在升高的CO2诱导的气孔关闭和碳酸氢盐激活S型阴离子通道电流中受到损害。与野生型相比，当生长在升高的CO2中时，它们不能表现出气孔密度和指数的降低。然而，与野生型一样，BIG突变体在暴露于升高的CO2时显示气孔开放抑制。BIG突变体还显示野生型气孔开度对关闭诱导刺激脱落酸（阿坝）的响应。我们的研究结果表明，BIG是一个信号成分参与升高CO2介导的气孔发育的控制。在CO2对气孔开度的调控中，BIG仅在CO2浓度升高引起的气孔关闭过程中起作用，而在CO2浓度升高抑制气孔开度的过程中不起作用。这些数据表明，在分子水平上，CO2介导的抑制气孔开放和促进气孔关闭信号通路是可分离的，BIG代表了这两种CO2介导的反应中的区别元素。
We conducted an infrared thermal imaging‐based genetic screen to identify Arabidopsis mutants displaying aberrant stomatal behavior in response to elevated concentrations of CO 2. This approach resulted in the isolation of a novel allele of the Arabidopsis BIG locus (At3g02260) that we have called CO 2 insensitive 1 (cis1). BIG mutants are compromised in elevated CO 2‐induced stomatal closure and bicarbonate activation of S‐type anion channel currents. In contrast with the wild‐type, they fail to exhibit reductions in stomatal density and index when grown in elevated CO 2. However, like the wild‐type, BIG mutants display inhibition of stomatal opening when exposed to elevated CO 2. BIG mutants also display wild‐type stomatal aperture responses to the closure‐inducing stimulus abscisic acid (ABA). Our results indicate that BIG is a signaling component involved in the elevated CO 2‐mediated control of stomatal development. In the control of stomatal aperture by CO 2, BIG is only required in elevated CO 2‐induced closure and not in the inhibition of stomatal opening by this environmental signal. These data show that, at the molecular level, the CO 2‐mediated inhibition of opening and promotion of stomatal closure signaling pathways are separable and BIG represents a distinguishing element in these two CO 2‐mediated responses.
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影响因子：
64.8
作者：
Engineer, Cawas B.;Ghassemian, Majid;Anderson, Jeffrey C.;Peck, Scott C.;Hu, Honghong;Schroeder, Julian I.
通讯作者：
Schroeder, Julian I.
RAF样激酶HT1基因中的显性和隐性突变完全破坏了拟南芥中对CO2的气孔反应。
影响因子：
6.9
作者：
Hashimoto-Sugimoto M;Negi J;Monda K;Higaki T;Isogai Y;Nakano T;Hasezawa S;Iba K
通讯作者：
Iba K
影响因子：
20.5
作者：
Engineer CB;Hashimoto-Sugimoto M;Negi J;Israelsson-Nordström M;Azoulay-Shemer T;Rappel WJ;Iba K;Schroeder JI
通讯作者：
Schroeder JI
影响因子：
4.6
作者：
Balcerowicz, Martin;Ranjan, Aashish;Hoecker, Ute
通讯作者：
Hoecker, Ute
影响因子：
10.5
作者：
Gil, P;Dewey, E;Chory, J
通讯作者：
Chory, J