miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1.
miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1.
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miR-148b-3p 通过靶向 cullin 相关和 neddylation 解离 1 促进雪旺细胞迁移
DOI：
10.4103/1673-5374.184504
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发表时间：
2016-06
影响因子：
6.1
通讯作者：
Zhou SL
中科院分区：
文献类型：
--
作者：
Qian TM;Zhao LL;Wang J;Li P;Qin J;Liu YS;Yu B;Ding F;Gu XS;Zhou SL
MicroRNAs （miRNAs）是一种小的非编码rna，在多种生物过程中负向调节基因表达。然而，mirna对雪旺细胞的调控作用仍然知之甚少。先前的微阵列分析结果表明，坐骨神经交易后miRNA表达发生改变，从而影响雪旺细胞的增殖和迁移。本研究探讨了miR-148b-3p是否可以通过直接靶向cullin-associated and neddyylation -dissociated 1 （Cand1）来调节雪旺细胞的迁移。上调miR-148b-3p的表达促进了雪旺细胞的迁移，而沉默miR-148b-3p则抑制了体外雪旺细胞的迁移。进一步的实验证实，Cand1是miR-148b-3p的直接靶点，Cand1敲低逆转了miR-148b-3p抑制剂对雪旺细胞迁移的抑制。这些结果表明，miR-148b-3p通过在体外直接靶向Cand1促进了雪旺细胞的迁移。
MicroRNAs (miRNAs) are small, non-coding RNAs that negatively adjust gene expression in multifarious biological processes. However, the regulatory effects of miRNAs on Schwann cells remain poorly understood. Previous microarray analysis results have shown that miRNA expression is altered following sciatic nerve transaction, thereby affecting proliferation and migration of Schwann cells. This study investigated whether miR-148b-3p could regulate migration of Schwann cells by directly targeting cullin-associated and neddylation-dissociated 1 (Cand1). Up-regulated expression of miR-148b-3p promoted Schwann cell migration, whereas silencing of miR-148b-3p inhibited Schwann cell migration in vitro. Further experiments confirmed that Cand1 was a direct target of miR-148b-3p, and Cand1 knockdown reversed suppression of the miR-148b-3p inhibitor on Schwann cell migration. These results suggested that miR-148b-3p promoted migration of Schwann cells by directly targeting Cand1 in vitro.