Human Polymorphonuclear Neutrophil Responses to Burkholderia pseudomallei in Healthy and Diabetic Subjects
Human Polymorphonuclear Neutrophil Responses to Burkholderia pseudomallei in Healthy and Diabetic Subjects
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DOI：
10.1128/iai.00503-08
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发表时间：
2009-01-01
影响因子：
3.1
通讯作者：
Lertmemongkolchai, Ganjana
中科院分区：
文献类型：
--
作者：
Chanchamroen, Sujin;Kewcharoenwong, Chidchamai;Lertmemongkolchai, Ganjana
类鼻疽病的主要诱发因素是糖尿病，但没有研究免疫学机制来解释这一点。在这项研究中，多形核中性粒细胞（PMN）响应类鼻疽伯克霍尔德氏菌，类鼻疽的病原体，在健康和糖尿病泰国受试者的流式细胞术测定。结果表明，B.与沙门氏菌血清型鼠伤寒和大肠杆菌相比，假鼻疽杆菌显示出减少的PMNs摄取。此外，细胞内存活的B。在整个24小时内检测到假鼻疽，表明B的内在抗性。假鼻疽致人死亡此外，糖尿病受试者的中性粒细胞显示受损的吞噬B。假鼻疽，响应于白细胞介素-8的迁移减少，以及不能延迟凋亡。这些数据表明，B.类鼻疽本质上抵抗PMN的吞噬作用和杀伤作用。这些观察结果，连同受损的迁移和细胞凋亡的糖尿病，可能解释宿主易感性类鼻疽。
The major predisposing factor for melioidosis is diabetes mellitus, but no immunological mechanisms have been investigated to explain this. In this study, polymorphonuclear neutrophil (PMN) responses to Burkholderia pseudomallei, the causative agent of melioidosis, in healthy and diabetic Thai subjects were determined by flow cytometry. The results showed that B. pseudomallei displayed reduced uptake by PMNs compared to Salmonella enterica serovar Typhimurium and Escherichia coli. Additionally, intracellular survival of B. pseudomallei was detected throughout a 24-h period, indicating the intrinsic resistance of B. pseudomallei to killing by PMNs. Moreover, PMNs from diabetic subjects displayed impaired phagocytosis of B. pseudomallei, reduced migration in response to interleukin-8, and an inability to delay apoptosis. These data show that B. pseudomallei is intrinsically resistant to phagocytosis and killing by PMNs. These observations, together with the impaired migration and apoptosis in diabetes mellitus, may explain host susceptibility in melioidosis.