Excitation relaxation dynamics and energy transfer in pigment-protein complexes of a dinoflagellate revealed by ultrafast fluorescence spectroscopy
Excitation relaxation dynamics and energy transfer in pigment-protein complexes of a dinoflagellate revealed by ultrafast fluorescence spectroscopy
复制标题
超快荧光光谱揭示甲藻色素-蛋白质复合物的激发弛豫动力学和能量转移
DOI：
10.1007/s11120-016-0238-x
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发表时间：
2016
影响因子：
3.7
通讯作者：
Murakami A and Akimoto S
中科院分区：
文献类型：
--
作者：
Tanaka K; Iida S; Takaichi S;Mimuro M;Murakami A and Akimoto S
光合捕光复合物存在于水生光合生物中，含有多种类胡萝卜素和叶绿素。大多数光合甲藻具有两种类型的捕光天线复合物：多甲藻素（Peri）-叶绿素（Chl）a/c-蛋白，作为一种内在的类囊体膜复合物蛋白（iPCP），和水溶性的Peri-Chla-蛋白，作为一种外在的膜蛋白（sPCP）的类囊体的内表面上。Peri是一种独特的类胡萝卜素，具有八个C=C键和一个C=O键，这导致在绿色波长区域的特征吸收带。本文研究了光合沟鞭藻共生藻（Symbiodiniumsp.）中Peri在溶液中的激发弛豫动力学以及sPCP与类囊体膜的激发能量传递过程，研究了超快时间分辨荧光光谱。我们发现，在sPCP和iPCP中，Peri到Chla的能量转移分别发生在Peri S1态，时间常数分别为1.5 ps和400 fs，Chlc到Chla的能量转移发生在350-400 fs和1.8-2.6 ps的时间范围内。
Photosynthetic light-harvesting complexes, found in aquatic photosynthetic organisms, contain a variety of carotenoids and chlorophylls. Most of the photosynthetic dinoflagellates possess two types of light-harvesting antenna complexes: peridinin (Peri)-chlorophyll (Chl)a/c-protein, as an intrinsic thylakoid membrane complex protein (iPCP), and water-soluble Peri-Chla-protein, as an extrinsic membrane protein (sPCP) on the inner surface of the thylakoid. Peri is a unique carotenoid that has eight C=C bonds and one C=O bond, which results in a characteristic absorption band in the green wavelength region. In the present study, excitation relaxation dynamics of Peri in solution and excitation energy transfer processes of sPCP and the thylakoid membranes, prepared from the photosynthetic dinoflagellate,Symbiodiniumsp., are investigated by ultrafast time-resolved fluorescence spectroscopy. We found that Peri-to-Chlaenergy transfer occurs via the Peri S1state with a time constant of 1.5 ps or 400 fs in sPCP or iPCP, respectively, and that Chlc-to-Chlaenergy transfer occurs in the time regions of 350–400 fs and 1.8–2.6 ps.