Effect of A-scan rate and interscan interval on optical coherence angiography
Effect of A-scan rate and interscan interval on optical coherence angiography
复制标题
DOI：
10.1364/boe.409636
复制
发表时间：
2021-02-01
影响因子：
3.4
通讯作者：
Lee, Hsiang-Chieh
中科院分区：
文献类型：
--
作者：
Chen, Ting-Hao;Wu, Yi-Chun;Lee, Hsiang-Chieh
光学相干断层扫描血管造影术（OCTA）可以提供快速，体积，无创成像的组织微血管，而无需外源性造影剂。为了研究A扫描速率和扫描间隔时间如何影响OCTA的对比度和动态范围，我们开发了一种1.06 μ m扫频源OCT系统，使用两个光源实现100 kHz或200 kHz OCT。仔细调整系统设置后，在100 kHz和200 kHz模态之间实现了几乎相同的检测灵敏度。对5只小鼠进行耳部皮肤的OCTA。我们使用可变扫描间时间分析算法（VISTA）和指定的扫描协议，通过相关映射方法重建OCTA图像。对于相对较长的扫描间时间（例如，12.5 ms与200-kHz OCT的6.25 ms），OCTA可以识别更复杂的微血管网络。具有相同扫描间时间的OCTA图像集（例如，12.5 MS）进行比较。与其他成像方式相比，以100 kHz A扫描速率采集的OCTA图像显示了更精细的微血管。我们对不同A扫描速率和扫描间隔时间重建的OCTA图像的血管面积、总血管长度和连接密度的对比度进行了定量分析。(C)根据OSA开放获取出版协议的条款，2021年美国光学学会
Optical coherence tomography angiography (OCTA) can provide rapid, volumetric, and noninvasive imaging of tissue microvasculature without the requirement of exogenous contrast agents. To investigate how A-scan rate and interscan time affected the contrast and dynamic range of OCTA, we developed a 1.06-mu m swept-source OCT system enabling 100-kHz or 200-kHz OCT using two light sources. After system settings were carefully adjusted, almost the same detection sensitivity was achieved between the 100-kHz and 200-kHz modalities. OCTA of ear skin was performed on five mice. We used the variable interscan time analysis algorithm (VISTA) and the designated scanning protocol with OCTA images reconstructed through the correlation mapping method. With a relatively long interscan time (e.g., 12.5 ms vs. 6.25 ms for 200-kHz OCT), OCTA can identify more intricate microvascular networks. OCTA image sets with the same interscan time (e.g., 12.5 ms) were compared. OCTA images acquired with a 100-kHz A-scan rate showed finer microvasculature than did other imaging modalities. We performed quantitative analysis on the contrast from OCTA images reconstructed with different A-scan rates and interscan time intervals in terms of vessel area, total vessel length, and junction density. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement