Cloning and analysis of a cDNA encoding farnesyl diphosphate synthase from Artemisia annua
Cloning and analysis of a cDNA encoding farnesyl diphosphate synthase from Artemisia annua
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DOI：
10.1016/0378-1119(96)00054-6
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发表时间：
1996-06-26
期刊：
影响因子：
3.5
通讯作者：
Charlwood, BV
中科院分区：
文献类型：
--
作者：
Matsushita, Y;Kang, W;Charlwood, BV
从黄花蒿(Artemisia Annua)的cDNA文库中克隆了编码法尼基二磷酸(FPP)合成酶(FPP)的基因。序列分析表明，该基因编码343个氨基酸残基，计算相对分子质量为39420 kDa。推导出的氨基酸序列与其他植物、酵母和哺乳动物中的FPPS高度相似，并含有聚戊烯基合酶中的两个保守结构域，即FPPS、香叶基香叶基二磷酸合成酶和六烯基二磷酸合成酶。该基因在大肠杆菌中的表达显示了对FPPS的体外活性。
A cDNA encoding farnesyl diphosphate (FPP) synthase (FPPS) has been cloned from a cDNA library of Artemisia annua. The sequence analysis showed that the cDNA encoded a protein of 343 amino acid (aa) residues with a calculated molecular weight of 39 420 kDa. The deduced aa sequence of the cDNA was highly similar to FPPS from other plants, yeast and mammals, and contained the two conserved domains found in polyprenyl synthases including FPPS, geranylgeranyl diphosphate synthases and hexaprenyl diphosphate synthases. The expression of the cDNA in Escherichia coli showed enzyme activity for FPPS in vitro.