Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus
Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus
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DOI：
10.1016/s0140-6736(06)68231-7
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发表时间：
2006-03-04
期刊：
影响因子：
168.9
通讯作者：
Perdreau-Remington, F
中科院分区：
文献类型：
--
作者：
Diep, BA;Gill, SR;Perdreau-Remington, F
背景：USA300是耐甲氧西林金黄色葡萄球菌的克隆，是美国、加拿大和欧洲社区获得性感染的主要来源。方法采用随机散弹枪测序的方法，对耐多药的S金黄色葡萄球菌进行基因组测序，并与其他10株金黄色葡萄球菌进行比较，发现与S金黄色葡萄球菌密切相关的基因几乎全部集中在新的可移动遗传元件中。一些独特的基因参与了USA300的致病过程，包括Panton-valtine Leucoidin以及肠毒素Q和K的分子变体。USA300基因组最显著的特征是水平获得一个新的可移动的遗传元件，该遗传元件编码一个精氨酸脱亚胺酶途径和一个寡肽渗透酶系统，这可能有助于USA300的生长和生存。我们在其他S金黄色葡萄球菌菌株中没有检测到这种被称为精氨酸分解代谢活动元件(ACME)的元件。我们注意到ACME在S表皮中的高流行率，这表明ACME不仅从S表皮转移到USA300，而且这种元件赋予了这种普遍存在的人类皮肤共生的选择性优势。解释USA300获得了可移动的遗传元件，编码了可以增强适合性和致病性的抗性和毒力决定因素。
Background USA300, a clone of meticillin-resistant Staphylococcus aureus, is a major source of community-acquired infections in the USA, Canada, and Europe. Our aim was to sequence its genome and compare it with those of other strains of S aureus to try to identify genes responsible for its distinctive epidemiological and virulence properties.Methods We ascertained the genome sequence of FPR3757, a multidrug resistant USA300 strain, by random shotgun sequencing, then compared it with the sequences of ten other staphylococcal strains.Findings Compared with closely related S aureus, we noted that almost all of the unique genes in USA300 clustered in novel allotypes of mobile genetic elements. Some of the unique genes are involved in pathogenesis, including Panton-Valentine leucocidin and molecular variants of enterotoxin Q and K. The most striking feature of the USA300 genome is the horizontal acquisition of a novel mobile genetic element that encodes an arginine deiminase pathway and an oligopeptide permease system that could contribute to growth and survival of USA300. We did not detect this element, termed arginine catabolic mobile element (ACME), in other S aureus strains. We noted a high prevalence of ACME in S epidermidis, suggesting not only that ACME transfers into USA300 from S epidermidis, but also that this element confers a selective advantage to this ubiquitous commensal of the human skin.Interpretation USA300 has acquired mobile genetic elements that encode resistance and virulence determinants that could enhance fitness and pathogenicity.