In Vivo and In Vitro Protein-Peptidoglycan Interactions.
In Vivo and In Vitro Protein-Peptidoglycan Interactions.
复制标题
体内和体外蛋白质-肽聚糖相互作用。
DOI：
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发表时间：
2017
影响因子：
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通讯作者：
S. Peter Howard
中科院分区：
文献类型：
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作者：
Gang Li;S. Peter Howard
细菌已经发展了许多跨被膜系统来跨细菌被膜运输分子或组装细胞器。然而，细菌包膜含有刚性网状肽聚糖结构，可保护细胞免受渗透裂解。因此，跨包膜系统必须与肽聚糖屏障相互作用以产生肽聚糖支架的间隙或锚结构。在这里，我们描述了使用体内交联和体外共沉降来研究革兰氏阴性菌中蛋白质-肽聚糖相互作用的方法。特别是，我们解决重要的考虑，以确保所讨论的相互作用的特异性。
Bacteria have developed a number of trans-envelope systems to transport molecules or assemble organelles across bacterial envelopes. However, bacterial envelopes contain a rigid netlike peptidoglycan structure that protects cells from osmotic lysis. Trans-envelope systems thus must interact with the peptidoglycan barrier to generate gaps or anchor structures to the peptidoglycan scaffold. Here we describe methods to use in vivo cross-linking and in vitro co-sedimentation to study protein-peptidoglycan interactions in Gram-negative bacteria. In particular, we address important considerations to ensure the specificity of the interactions in question.