ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death
ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death
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DOI：
10.1016/j.bbrc.2008.11.021
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发表时间：
2009-01-02
影响因子：
3.1
通讯作者：
Berchtold, Martin W.
中科院分区：
文献类型：
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作者：
Hoj, Berit R.;la Cour, Jonas M.;Berchtold, Martin W.
ALG-2（凋亡相关基因-2编码蛋白）已被证明在多种人类肿瘤中上调，质疑其先前假定的促凋亡功能。本研究的目的是深入了解ALG-2在人类癌细胞中的作用。我们发现ALG-2下调诱导HeLa细胞在G2/M细胞周期阶段的积累，并增加早期凋亡和死亡细胞的数量。通过泛半胱天冬酶抑制剂zVAD-favorite的半胱天冬酶抑制减弱了ALG-2下调后死亡细胞量的增加。因此，我们的结果表明，ALG-2通过促进通过G2/M细胞周期阶段中的检查点而在HeLa细胞中具有抗凋亡功能（C）2008 Elsevier Inc. All rights reserved.
ALG-2 (apoptosis-linked gene-2 encoded protein) has been shown to be upregulated in a variety of human tumors questioning its previously assumed pro-apoptotic function. The aim of the present study was to obtain insights into the role of ALG-2 in human cancer cells. We show that ALG-2 downregulation induces accumulation of HeLa cells in the G2/M cell cycle phase and increases the amount of early apoptotic and dead cells. Caspase inhibition by the pan-caspase inhibitor zVAD-fmk attenuated the increase in the amount of dead cells following ALG-2 downregulation. Thus, our results indicate that ALG-2 has an anti-apoptotic function in HeLa cells by-facilitating the passage through checkpoints in the G2/M cell cycle phase (C) 2008 Elsevier Inc. All rights reserved.