Hydrophobic ionic liquid immoblizing cholesterol oxidase on the electrodeposited Prussian blue on glassy carbon electrode for detection of cholesterol
Hydrophobic ionic liquid immoblizing cholesterol oxidase on the electrodeposited Prussian blue on glassy carbon electrode for detection of cholesterol
复制标题
疏水离子液体将胆固醇氧化酶固定在玻碳电极电沉积普鲁士蓝上检测胆固醇
DOI：
10.1016/j.electacta.2012.11.119
复制
发表时间：
2013-02-15
影响因子：
6.6
通讯作者：
Lu, Xiaoquan
中科院分区：
文献类型：
--
作者：
Liu, Xiuhui;Nan, Zhihan;Lu, Xiaoquan
在疏水离子液体/水溶液界面上制备了一种新型胆固醇生物传感器。疏水IL薄膜不仅能从水溶液中富集胆固醇，还能固定化胆固醇氧化酶（ChOx）基质，从而发挥了信号放大作用。普鲁士蓝（PB）作为一种先进的传感材料，作为过氧化氢（H2O2）的有效低电位电子转移介质。利用电化学阻抗谱（EIS）和循环伏安谱（CV）对制备的IL-ChOx/PB/玻碳电极（GCE）进行了表征。在0.01 ~ 0.40 mM范围内对胆固醇呈线性响应，检出限为4.4 μ m。此外，考察了胆固醇在IL-Chox/PB/GCE电极上的动力学行为，提出了IL-Chox/PB/GCE电极的电催化机理。在疏水IL薄膜中固定化ChOx作为第一级电催化剂将胆固醇转化为H2O2，在GCE上的PB膜作为第二级电催化剂将生成的H2O2 2e（-）还原为H2O。(C) 2012 Elsevier Ltd.版权所有。
A novel cholesterol biosensor was fabricated on hydrophobic ionic liquid (IL)/aqueous solution interface. The hydrophobic IL thin film played a signal amplification role because it not only enriched the cholesterol from the aqueous solution, but also immobilized matrix for cholesterol oxidase (ChOx). Prussian blue (PB) as advanced sensing materials was used as effective low-potential electron transfer mediation toward hydrogen peroxide (H2O2). The fabricated IL-ChOx/PB/Glassy carbon electrode (GCE) was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammogram (CV), respectively. And it exhibited a linear response to cholesterol in the range of 0.01-0.40 mM with a detection limit of 4.4 mu M. In addition, the kinetics behavior of cholesterol at IL-Chox/PB/GCE electrode was examined, and the electrocatalytic mechanism was proposed as shown in first scheme. ChOx immobilized in hydrophobic IL thin film was used as the first electrocatalyst for the cholesterol into H2O2, and the PB film onto the GCE was used as the second electrocatalyst for the 2e(-) reduction of the produced H2O2 into H2O. (C) 2012 Elsevier Ltd. All rights reserved.