Multiple PolK (POLK) transcripts in mammalian testis
Multiple PolK (POLK) transcripts in mammalian testis
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DOI：
10.1016/j.dnarep.2004.10.006
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发表时间：
2005-03-02
期刊：
影响因子：
3.8
通讯作者：
Friedberg, EC
中科院分区：
文献类型：
--
作者：
Guo, C;Gao, T;Friedberg, EC
DNA聚合酶的Y家族能够在体外和体内进行跨损伤合成。编码Y家族成员Polk的小鼠/人基因（POIK/POLK）在小鼠/人睾丸中高度表达。用RT-PCR方法扩增Polk基因编码序列，从睾丸组织中鉴定出11种小鼠Polk和5种人POLK转录本。这种现象显然是PolK特有的，因为其他几种能够TLS的专门DNA聚合酶在睾丸中不表达功能性替代转录本。小鼠/人（PolK/POLK）多个转录本在睾丸中可编码多个Polkappa异构体。(C)2004 Elsevier B. V.保留所有权利。
The Y-family of DNA polymerases are capable of translesion synthesis both in vitro and in vivo. The mouse/human gene (POlK/POLK) that encodes the Y-family member Polk is highly expressed in mouse/human testis. Using RT-PCR to amplify the coding sequence of the Polk gene, 11 forms of mouse PolK and 5 forms of human POLK transcripts were identified from testis. This phenomenon is apparently specific to PolK since several other specialized DNA polymerases capable of TLS do not express functional alternative transcripts in the testis. The multiple mouse/human (PolK/POLK) transcripts may encode multiple Polkappa isoforms in testis. (C) 2004 Elsevier B.V. All rights reserved.