Sulfated polysaccharide from Cyclocarya paliurus enhances the immunomodulatory activity of macrophages
Sulfated polysaccharide from Cyclocarya paliurus enhances the immunomodulatory activity of macrophages
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青钱柳硫酸多糖增强巨噬细胞的免疫调节活性
DOI：
10.1016/j.carbpol.2017.07.009
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发表时间：
2017
影响因子：
11.2
通讯作者：
Xie Jianhua
中科院分区：
文献类型：
--
作者：
Yu Yue;Shen Mingyue;Wang Zhijun;Wang Yuanxing;Xie Mingyong;Xie Jianhua
以青钱柳（Cyclocarya paliurus）为原料，采用氯磺酸-吡啶法从青钱柳多糖中分离得到一种硫酸酯化多糖（S-CP 1 -8）。对S-CP 1 - 8进行化学鉴定，并与巨噬细胞RAW264.7进行培养，以评价其免疫调节活性。初步的理化分析确定阿拉伯糖（Ara）、半乳糖（Gal）和葡萄糖（Glc）是S-CP 1 -8的主要单糖，平均分子量（Mw）为970 kDa，含有取代度（DS）为0.12的硫酸盐。同时，S-CP 1 - 8处理后，RAW 264. 7细胞分泌肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β和IL-6的水平明显升高，并呈剂量依赖性。与天然青钱柳多糖（CP）相比，S-CP 1 - 8能显著增强巨噬细胞的一氧化氮（NO）释放和吞噬活性。结果表明，S-CP 1 - 8对RAW264.7巨噬细胞的免疫调节作用较CP组增强。S-CP 1 - 8可作为潜在的免疫调节剂用于辅助药物或功能性食品。
In this study, a sulfated polysaccharide (S-CP1-8) was obtained fromCyclocarya paliurusby chlorosulfonic acid-pyridine method. S-CP1-8was chemical characterized and cultured with macrophage RAW264.7 cells to evaluate its immunomodulatory activity. Preliminary physicochemical analysis identified arabinose (Ara), galactose (Gal) and glucose (Glc) were the major monosaccharides of S-CP1-8, with average Molecular weight (Mw) of 970 kDa and contained sulfate with degree of substitution (DS) of 0.12. Meanwhile, our findings demonstrated that tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 secretion levels were significantly increased by S-CP1-8treatment in RAW264.7 cells in a dose-dependent manner. Moreover, nitric oxide (NO) release and phagocytic activities of macrophages were significantly enhanced by the treatment of S-CP1-8compared with nativeCyclocarya paliuruspolysaccharide (CP). The results indicated that S-CP1-8could enhance the immunomodulatory effects on RAW264.7 macrophages, as comparison with CP group. S-CP1-8could serve as potential immunomodulatory agents to be used as complementary medicine or functional foods.