Anti-nutritional effects of a moderate dose of soybean agglutinin in the rat
Anti-nutritional effects of a moderate dose of soybean agglutinin in the rat
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DOI：
10.1080/00039420310001594414
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发表时间：
2003-08-01
影响因子：
2
通讯作者：
Huang, CH
中科院分区：
文献类型：
--
作者：
Li, ZT;Li, DF;Huang, CH
本研究旨在研究中等剂量纯化大豆凝集素对大鼠生产性能和氮消化率的影响，以及对小肠和胰腺蛋白质、DNA和RNA含量的影响。将24只SD大鼠随机分为4组，进行为期10天的氮平衡实验。四组大鼠喂食7 g酪蛋白-玉米淀粉基饮食或补充有0.1、0.2或0.4 mg/g纯化大豆凝集素的类似饮食。将所有实验饮食调整至相同的营养水平。大豆凝集素的剂量对大鼠的生产性能没有显著影响。大豆凝集素在日粮中的掺入降低了蛋白质的表观消化率和通过增加氮从粪便和尿液中的损失而降低了对日粮蛋白质的利用。与对照组相比，在饲料中以0.4 mg/g的水平饲喂大豆凝集素的大鼠的新鲜胰腺重量增加，但胰腺的干重和蛋白质含量在四组之间没有差异。但随着大豆凝集素水平的提高，胰腺DNA和RNA含量有增加的趋势。大豆凝集素对空肠重量及其蛋白质、DNA和RNA含量无显著影响，但空肠干重和RNA含量有随日粮中大豆凝集素水平升高而增加的趋势。因此，在大鼠日粮中添加适量的纯化大豆凝集素对氮消化率、氮存留率和氮平衡等消化功能有负面影响。随着大豆凝集素水平的增加，效果变得更加明显。同时，随着饲料中大豆凝集素掺入量的增加，胰腺肥大。
This study was conducted to investigate the effects of a moderate dose of purified soybean agglutinin on performance and nitrogen digestibility in rats as well as to determine its effects on the protein, DNA and RNA content of the small intestine and pancreas. Twenty-four Sprague-Dawley rats were randomly allotted into one of four groups for a 10-day nitrogen balance experiment. The four groups of rats were fed 7 g of a casein-cornstarch based diet or a similar diet supplemented with 0.1, 0.2 or 0.4 mg/g purified soybean agglutinin. All experimental diets were adjusted to an identical nutrient level. Dose of soybean agglutinin had no significant effect on rat performance. Incorporation of soybean agglutinin in the diet reduced apparent protein digestibility and the utilization of dietary protein by increasing nitrogen loss from the faeces and urine. Fresh pancreatic weight increased in rats fed soybean agglutinin at a level of 0.4 mg/g in the diet compared to the control, but the dry pancreatic weight and the protein content of the pancreas did not differ among the four groups. However the DNA and RNA content of the pancreas had a tendency to increase with a higher level of soybean agglutinin. The weight of the jejunum and its protein, DNA and RNA content were not significantly affected by soybean agglutinin, but the dry weight and the RNA of the jejunum tended to increase with higher levels of soybean agglutinin in the diet. In conclusion, purified soybean agglutinin, at moderate levels in the rats diet, had negative effects on digestive function, such as nitrogen digestibility, nitrogen retention and nitrogen balance. As the level of soybean agglutinin increased, the effects became more pronounced. Meanwhile, hypertrophy of the pancreas was observed with higher doses of soybean agglutinin incorporation in the diets.