Audiograms, gap detection thresholds, and frequency difference limens in cannabinoid receptor 1 knockout mice.
Audiograms, gap detection thresholds, and frequency difference limens in cannabinoid receptor 1 knockout mice.
复制标题
DOI：
10.1016/j.heares.2015.09.013
复制
发表时间：
2016-02
期刊：
影响因子：
2.8
通讯作者：
Dent ML
中科院分区：
文献类型：
--
作者：
Toal KL;Radziwon KE;Holfoth DP;Xu-Friedman MA;Dent ML
大麻素受体 1 (CB1R) 在听觉通路的多个阶段被发现，但其在听力中的作用尚不清楚。对 CB1R 敲除小鼠的听力进行了测量，并与野生型小鼠进行了比较。使用与之前实验相同的方法和刺激，使用操作条件反射和恒定刺激的心理物理学方法来测量受过训练的小鼠的听力图、间隙检测阈值和频率差阈值。与野生型小鼠相比，CB1R 基因敲除小鼠的听力图显示频率高于 8 kHz 的缺陷。相对于野生型小鼠，CB1R 基因敲除显示出检测低通噪声爆发间隙的增强，但对于其他噪声条件也相似。最后，通过频差阈值任务测量，两组小鼠的频率辨别能力没有差异。这些实验表明 CB1R 参与听觉处理，并为未来的生理实验奠定基础。
The cannabinoid receptor 1 (CB1R) is found at several stages in the auditory pathway, but its role in hearing is unknown. Hearing abilities were measured in CB1R knockout mice and compared to those of wild-type mice. Operant conditioning and the psychophysical Method of Constant Stimuli were used to measure audiograms, gap detection thresholds, and frequency difference limens in trained mice using the same methods and stimuli as in previous experiments. CB1R knockout mice showed deficits at frequencies above 8 kHz in their audiograms relative to wild-type mice. CB1R knockouts showed enhancements for detecting gaps in low-pass noisebursts relative to wild-type mice, but were similar for other noise conditions. Finally, the two groups of mice did not differ in their frequency discrimination abilities as measured by the frequency difference limens task. These experiments suggest that the CB1R is involved in auditory processing and lay the groundwork for future physiological experiments.