Expression of group II phospholipase A2 in the human gastrointestinal tract.
Expression of group II phospholipase A2 in the human gastrointestinal tract.
复制标题
II 族磷脂酶 A2 在人胃肠道中的表达。
DOI：
--
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
M. Kallajoki
中科院分区：
文献类型：
--
作者：
T. Nevalainen;Grönroos Jm;M. Kallajoki
背景
已经表明II族磷脂酶A2（PLA 2-II）在炎症中起重要作用，但是该酶的细胞来源在很大程度上是未知的。
实验设计
在蛋白质和mRNA水平上分析人胃肠道中PLA 2-II的表达。分析了正常组织和几种常见胃肠道疾病患者的标本。
结果
PLA 2-II mRNA的表达被认为是在潘氏细胞，但没有在任何其他类型的肠道细胞。血管壁显示PLA 2-II免疫反应性，但在PLA 2-II mRNA的原位杂交阴性。
结论
得出的结论是，nonpancreatic组II PLA 2的合成和存储的潘氏细胞，而其他类型的胃肠道细胞似乎无法合成这种酶。血管结构中的阳性免疫反应可能反映了循环PLA 2-II进入血管壁，而不是局部产生的酶。
BACKGROUND
It has been suggested that group II phospholipase A2 (PLA2-II) plays an essential role in inflammation, but the cellular source(s) of the enzyme is (are) largely unknown.
EXPERIMENTAL DESIGN
Expression of PLA2-II was analyzed in human gastrointestinal tract at both protein and mRNA levels. Both normal tissues and specimen from patients with a few different common gastrointestinal disorders were analyzed.
RESULTS
Expression of PLA2-II mRNA was seen in the Paneth cells but not in any other cell type of the intestinal tract. Blood vessel walls showed PLA2-II immunoreactivity but were negative in in situ hybridization for PLA2-II mRNA.
CONCLUSIONS
It was concluded that nonpancreatic group II PLA2 is synthesized and stored by Paneth cells, whereas other cell types of the gastrointestinal tract seem incapable of synthesis of this enzyme. The positive immunoreaction in vascular structures may reflect the entry of circulating PLA2-II into vessel walls rather than local production of the enzyme.