Varietal Differences in the Ability of Callus Formation and Plant Regeneration from Mature Embryo in Barley (Hordeum vulgare L.)
Varietal Differences in the Ability of Callus Formation and Plant Regeneration from Mature Embryo in Barley (Hordeum vulgare L.)
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DOI：
10.1270/jsbbs1951.41.571
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发表时间：
1991-12
期刊：
影响因子：
2.4
通讯作者：
M. Taniguchi;S. Enomoto;T. Komatsuda;Mamiko Nakajima;K. Ohyama
中科院分区：
文献类型：
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作者：
M. Taniguchi;S. Enomoto;T. Komatsuda;Mamiko Nakajima;K. Ohyama
以203个日本和国外引进的大麦品种为材料，研究了成熟胚离体愈伤组织的形成及植株再生。其中包括176个六棱和27个二棱基因型。从种子上分离的成熟胚接种在含2 mg/l 2，4-二氯苯氧乙酸（2，4-D）的改良N6培养基上。有193个品种产生了愈伤组织，但大多数愈伤组织质地柔软、水分多、半透明，增殖能力差。次生愈伤组织可以再生植株，特别是一些二棱基因型。2，4-D浓度增加到5 mg/l时，对次生愈伤组织的生长有促进作用。对14个品种进行了植株再生观察，其中6个为六棱品种，8个为二棱品种。所用的一些品种，特别是二棱品种，可能有助于大麦成熟胚培养系统的建立。
In vitro callus formation by mature embryo explants, and subsequent plant regeneration were investigated using 203 Japanese and introduced varieties of barley (Hordeum vulgare L.). These included 176 six-rowed and 27 two-rowed genotypes. Mature embryos detached from seeds were inoculated on the modified N6 medium containing 2 mg/l 2, 4-dichlorophenoxyacetic acid (2, 4-D). Callus was produced in 193 varieties, although most of the calli were soft, watery, translucent and poor in proliferation. Plants could be regenerated from secondary callus especially with some two-rowed genotypes. The growth of secondary callus was promoted by increasing 2, 4-D concentration to 5 mg/l. Plant regeneration was observed in 14 varieties including 6 six-rowed and 8 two-rowed ones. Some of the varieties used, especially the two-rowed ones: may be useful for developing the mature embryo culture system in barley.