1H MRS detects polyunsaturated fatty acid accumulation during gene therapy of glioma:: Implications for the in vivo detection of apoptosis
1H MRS detects polyunsaturated fatty acid accumulation during gene therapy of glioma:: Implications for the in vivo detection of apoptosis
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DOI：
10.1038/15279
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发表时间：
1999-11-01
期刊：
影响因子：
82.9
通讯作者：
Kauppinen, RA
中科院分区：
文献类型：
--
作者：
Hakumäki, JM;Poptani, H;Kauppinen, RA
1.1 高倍视野（HPF）可检测到TUNEL阳性凋亡细胞核。在 GCV 处理的第 4 天，这个数字明显更高（每个 HPF 8.1±1.7，P < 0.05），忽略仅由凋亡细胞组成的区域（图 1d 和 e），表明广泛的凋亡2。到第 8 天，组织细胞数量减少，含有分散的肿瘤细胞和巨噬细胞，以及形成疤痕的星形胶质细胞（图 1f 和 g）。肿瘤的透射电子显微镜切片的特征是第 4 天时肿瘤细胞内出现凋亡小体和数量增多的脂滴（直径 0.2–2.0 µm）（未经治疗的肿瘤每个视野为 43±5 与 23±3，P < 0.05）。这些液滴中明显较高比例的嗜锇性密集，表明第 4 天存在多不饱和（图 2c）（未处理时为 37±3% 对比 19±8%，P < 0.05）。
1.1 TUNEL-positive apoptotic nuclei could be detected per highpower field (HPF). At day 4 of GCV treatment this number was significantly higher (8.1±1.7 per HPF, P< 0.05), ignoring regions consisting of only apoptotic cells (Fig. 1d and e), demonstrating widespread apoptosis2. By day 8 tissue became less cellular, containing scattered tumor cells and macrophages, as well as scar-forming astroglial cells (Fig. 1f and g). Transmission electron microscopy sections of tumors were characterized by apoptotic bodies and an elevated number of lipid droplets (diameter 0.2–2.0 µm) within tumor cells at day 4 (43±5 versus 23±3 per field in untreated tumors, P< 0.05). A significantly higher proportion of these droplets was densely osmiophilic, indicating polyunsaturation (Fig. 2c) on day 4 (37±3% vs. 19±8% in untreated, P< 0.05).