JACK-BEAN UREASE (EC 3.5.1.5) - METALLOENZYME - SIMPLE BIOLOGICAL ROLE FOR NICKEL
JACK-BEAN UREASE (EC 3.5.1.5) - METALLOENZYME - SIMPLE BIOLOGICAL ROLE FOR NICKEL
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DOI：
10.1021/ja00847a045
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发表时间：
1975-01-01
影响因子：
15
通讯作者：
ZERNER, B
中科院分区：
文献类型：
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作者：
DIXON, NE;GAZZOLA, C;ZERNER, B
5.07 0.934 I. 43 8.533 se。lgd 0.414 0.427 5.8 I751.83？0.430 0.327 4.28 g 99.2 h 0.655< 0.006< 0.05 a以原始比酶活性的百分比表示。6[蛋白结合抑制剂]，单位为umol/ml；[蛋白质]，单位为g/ml。最大抑制酶。^在38度时重新激活5分钟。e在38度时重新激活15分钟。/最大限度地抑制酶，在38℃下重新激活2小时，在4℃时在磷酸缓冲液中透析（4个循环）。在38度时重新激活10分钟。最大限度抑制酶，在38℃下对n -乙基morpholine缓冲液透析18小时，然后在4 （6 X 250 vol）下对相同的缓冲液透析。
5.07 0.934 I. 4I3 8.533 se. lgd 0.414 0.427 5.8 I751.83? 0.430 0.327 4.28 g 99.2 h 0.655< 0.006< 0.05 a Expressed as a percentage of the original specific enzymatic activity. 6 [Protein-bound inhibitor] in units of umol/ml;[protein] in units of g/ml. cMaximally inhibited enzyme.^ Reactivated for 5 min at 38. e Reactivated for 15 min at 38./Maximally inhibited enzyme, reactivated for 2 hr at 38 and dialyzed at 4 in the phos-phate buffer (four cycles). 8Reactivated for 10 min at 38. hMax-imally inhibited enzyme, dialyzed for 18 hr at 38 against the N-ethylmorpholine buffer, and then against the same buffer at 4 (6 X 250 vol).