Characterisation of the variation of mouse brain proteome by two-dimensional electrophoresis
Characterisation of the variation of mouse brain proteome by two-dimensional electrophoresis
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DOI：
10.1016/j.jprot.2011.03.006
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发表时间：
2011-05-16
影响因子：
3.3
通讯作者：
Janaky, Tamas
中科院分区：
文献类型：
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作者：
Foeldi, Istvan;Mueller, Geza;Janaky, Tamas
神经蛋白质组学的目的是在蛋白质水平上研究神经系统的分子组织。双向电泳是定量蛋白质组学中最常用的技术。本研究的目的是使用小鼠脑组织评估该蛋白质组学平台的实验和生物学变化。小鼠是最常用的实验室动物，用于模拟人类疾病或研究候选药物或治疗的效果。实验设计在定量蛋白质组学中起着至关重要的作用，因此理解和最小化变量至关重要。我们的研究结果表明，技术的方差占主导地位，在小鼠脑的总变异和遗传背景的影响可以忽略不计。这些结果也证实了使用小鼠脑进行蛋白质组学研究的预期变化，因此它们应该对其他蛋白质组学实验室未来的实验设计有用。(C)2011 Elsevier B.V.保留所有权利。
Neuroproteomics is aimed to study the molecular organisation of the nervous system at the protein level. Two-dimensional electrophoresis is the most frequently used technique in quantitative proteomics. The aim of this study was to assess the experimental and biological variations on this proteomic platform using mouse brain tissue. Mice are the most generally used lab animals for modelling human disease or investigating the effect of a drug-candidate or a treatment. Experimental design plays a crucial role in quantitative proteomics, hence understanding and minimizing the variables is essential. Our results indicate that the technical variance dominantly contributes to the total variance in mouse brain and the genetic background has a negligible effect on the total variation. The results also characterise the anticipated variation using mouse brain for proteomic study hence they should be useful for future experimental design in other proteomics laboratories. (C) 2011 Elsevier B.V. All rights reserved.