CD4+CD8+ and CD8α+β- T lymphocytes in human small intestinal lamina propria
CD4+CD8+ and CD8α+β- T lymphocytes in human small intestinal lamina propria
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DOI：
10.1097/00042737-199804000-00009
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发表时间：
1998-04-01
影响因子：
2.1
通讯作者：
O'Farrelly, C
中科院分区：
文献类型：
--
作者：
Abuzakouk, M;Carton, J;O'Farrelly, C
目的检测正常人小肠粘膜固有层T淋巴细胞亚群CD 8的表达。方法取正常人小肠粘膜固有层和上皮细胞，制备单细胞悬液。结果除典型的CD 4+和CD 8 + T淋巴细胞外，固有层T淋巴细胞中相当一部分为CD 4+和CD 8+或“双阳性”T淋巴细胞，其余均为CD 4+和CD 8 + T淋巴细胞。该群体在相同患者的上皮层中显著较低（平均7%，范围3- 21%，P < 0.007）。三色流式细胞术分析显示，固有层中双阳性T细胞上的CD 8分子的表达仅限于CD 8 α链。此外，在CD 8（+）群体中，仅表达α链的CD 8（+）T细胞在固有层中的数量（平均35%，范围14-54%）多于在上皮层中的数量（平均18%，范围5- 37%，P < 0.02）。在固有层中未检测到NK（CD 56（+））细胞，并且检测到少量γ δ TCR+ T淋巴细胞（平均2%，范围0.5-6.6%）（平均8%，范围0.2- 14%，结论小肠固有层中存在大量CD 8 β链阴性的CD 4 + CD 8 α + T淋巴细胞。这些细胞形成了比上皮层T细胞库更重要的固有层组成部分，并可能在肠道免疫调节中具有独特的功能。(C)1998 Lippincott-Raven出版社。
Objective To examine CD8 expression by T-lymphocyte subpopulations from disease-free human lamina propria.Methods Single-cell suspensions were prepared from the epithelial layer and the lamina propria of small intestinal biopsies obtained endoscopically from disease-free patients. Monoclonal antibodies against CD3, CD4, CD8, CD56, CD8 alpha beta, CD8 alpha, TCR alpha beta and TCR gamma delta were used for dual and three-colour flow cytometric analysis.Results In addition to classical CD4(+) and CD8(+) T lymphocytes a substantial proportion of lamina propria T lymphocytes were CD4(+)CD8(+) or 'double positive' (mean 14% range 4-26%), This population was significantly lower in the epithelial layer of the same patients (mean 7%, range 3-21%, P < 0.007). Three-colour flow cytometric analysis revealed that expression of the CD8 molecule on double positive T cells in the lamina propria was limited to the CD8 alpha chain. Furthermore, of the CD8(+) population, CD8(+) T cells which only expressed the alpha chain were present in greater numbers in the lamina propria (mean 35%, range 14-54%) than in the epithelial layer (mean 18%, range 5-37%, P < 0.02). NK (CD56(+)) cells were not detected and few gamma delta TCR+ T lymphocytes were detected in the lamina propria (mean 2%, range 0.5-6.6%) when compared with the epithelial layer (mean 8%, range 0.2-14%, P< 0.008).Conclusion A significant population of CD4(+)CD8 alpha(+) T lymphocytes which are CD8 beta chain negative have been detected in the intestinal lamina propria. These cells form a more significant component of the lamina propria than the epithelial layer T-cell repertoire and may have a unique function in intestinal immunoregulation. (C) 1998 Lippincott-Raven Publishers.