Production of an emericellin and its analogues as fungal biological responses for Shimbu-to extract
Production of an emericellin and its analogues as fungal biological responses for Shimbu-to extract
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生产艾美西林及其类似物作为 Shimbu-to 提取物的真菌生物反应
DOI：
10.1007/s11418-017-1156-8
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发表时间：
2017
影响因子：
3.3
通讯作者：
Hosoe Tomoo
中科院分区：
文献类型：
--
作者：
Inoue Nobuhiro;Wakana Daigo;Takeda Hisashi;Yaguchi Takashi;Hosoe Tomoo
本研究检测了真菌代谢产物的产生，作为对汉方药物的生物反应。Shimbu-to（SMB）是一种汉方药，由五种草药成分组成：牡丹根（Shakuyaku），生姜（Shokyo），加工过的乌头根（Bushi），Poriasclerotium（Bukuryo），和Lancearhizomes（Sojutsu）。在添加SMB提取物的马铃薯葡萄糖肉汤中培养的真菌构巢曲霉CBS 112.46的高效液相色谱（HPLC）分析显示，emericellin（2）为主峰，新的咕吨酮类似物24-羟基沙米咕吨酮（1）、沙米咕吨酮（3）、表沙米咕吨酮（4）、前沙米咕吨酮（5）和变异咕吨酮A（6）为次峰。通过1D-NMR、2D-NMR和MS数据的详细分析确定了1的结构。结果表明，丹参提取物对A. nidulans。进一步的研究表明，葡萄糖诱导A. nidulansin呈浓度依赖性。
This research examined the production of fungal metabolites as a biological response to Kampo medicines. Shimbu-to (SMB) is a Kampo medicine composed of five herbal components: peony root (Shakuyaku), ginger (Shokyo), processed aconite root (Bushi),Poriasclerotium (Bukuryo), andAtractylodes lancearhizomes (Sojutsu). High-performance liquid chromatography (HPLC) analysis of the fungusAspergillus nidulansCBS 112.46 incubated in potato dextrose broth supplemented with SMB extract revealed emericellin (2) as the major peak and new xanthone analogues 24-hydroxyshamixanthone (1), shamixanthone (3), epishamixanthone (4), pre-shamixanthone (5), and variecoxanthone A (6) as minor peaks. The structure of1was determined by detailed analysis of 1D-NMR, 2D-NMR, and MS data. The results suggest that SMB extract regulates the biosynthesis of emericellin and its analogues inA. nidulans. Further investigations revealed that glucose induces the biosynthesis of emericellin and its analogues inA. nidulansin a concentration-dependent manner.