Divergence in centromere structure distinguishes related genomes in Coix lacryma-jobi and its wild relative
Divergence in centromere structure distinguishes related genomes in Coix lacryma-jobi and its wild relative
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着丝粒结构的差异区分了薏苡及其野生近缘种的相关基因组
DOI：
10.1007/s00412-009-0239-z
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发表时间：
2010-02-01
期刊：
影响因子：
1.6
通讯作者：
Jin, Weiwei
中科院分区：
文献类型：
--
作者：
Han, Yonghua;Wang, Guixiang;Jin, Weiwei
了解着丝粒的组成和结构对于了解着丝粒如何发挥其功能作用至关重要。在这里，我们报告了一个着丝粒相关的细菌人工染色体克隆从薏苡库的序列。两个Ty 3/gypsy类反转录转座子，C. lacryma-jobi（CRC）和C. lacryma-jobi和一个单位长度为153 bp的着丝粒特异性串联重复序列。CRC与禾本科植物中报道的着丝粒特异性反转录转座子高度同源。在153-bp的卫星重复序列中的一个80-bp的DNA区域被发现是玉米，水稻和珍珠粟的着丝粒卫星重复序列的保守区域。荧光原位杂交结果表明，这三个重复序列分别位于两个C.和薏苡。而153 bp的随体重复序列仅在C.水生植物用抗水稻CENH 3的抗体进行的免疫染色表明，153-bp的卫星重复序列和CRC可能是功能性着丝粒的主要成分，但并非所有的153-bp的卫星重复序列或CRC序列都与CENH 3相关。研究了C.对多倍体化过程中的lacryma-jobi现象进行了讨论。
Knowledge about the composition and structure of centromeres is critical for understanding how centromeres perform their functional roles. Here, we report the sequences of one centromere-associated bacterial artificial chromosome clone from a Coix lacryma-jobi library. Two Ty3/gypsy-class retrotransposons, centromeric retrotransposon of C. lacryma-jobi (CRC) and peri-centromeric retrotransposon of C. lacryma-jobi, and a (peri)centromere-specific tandem repeat with a unit length of 153 bp were identified. The CRC is highly homologous to centromere-specific retrotransposons reported in grass species. An 80-bp DNA region in the 153-bp satellite repeat was found to be conserved to centromeric satellite repeats from maize, rice, and pearl millet. Fluorescence in situ hybridization showed that the three repetitive sequences were located in (peri-)centromeric regions of both C. lacryma-jobi and Coix aquatica. However, the 153-bp satellite repeat was only detected on 20 out of the 30 chromosomes in C. aquatica. Immunostaining with an antibody against rice CENH3 indicates that the 153-bp satellite repeat and CRC might be both the major components for functional centromeres, but not all the 153-bp satellite repeats or CRC sequences are associated with CENH3. The evolution of centromeric repeats of C. lacryma-jobi during the polyploidization was discussed.