Natural suppressor (NS) cells found in the spleen of neonatal mice and adult mice given total lymphoid irradiation (TLI) express the null surface phenotype.
Natural suppressor (NS) cells found in the spleen of neonatal mice and adult mice given total lymphoid irradiation (TLI) express the null surface phenotype.
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DOI：
10.4049/jimmunol.132.1.101
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发表时间：
1984-01
影响因子：
4.4
通讯作者：
A. Oseroff;S. Okada;S. Strober
中科院分区：
文献类型：
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作者：
A. Oseroff;S. Okada;S. Strober
我们研究了出生后新生小鼠和全淋巴照射（TLI）后成年小鼠脾脏中短暂存在的混合白细胞反应（MLR）抑制细胞的表面标记物。大约80%的脾脏单核细胞，在出生后的头几天或TLI后，既不表达Thy-1抗原，也不表达表面免疫球蛋白（Ig）。30天后，只有不到20%的单核细胞具有这种零表型。利用筛选技术，我们发现MLR的抑制因子局限于零细胞群。无效抑制细胞不是巨噬细胞，因为它们不携带抗mac -1和F4/80单克隆抗体识别的巨噬细胞标记物。此外，抑制细胞没有非特异性酯酶染色，也没有牢固地粘附在塑料或玻璃上。tli处理小鼠脾细胞在体外培养6 ~ 8周后仍保持抑制能力。在这段时间内，培养的抑制细胞没有形成成熟的T细胞、B细胞或巨噬细胞标记物。因此，抑制细胞似乎不是后一种细胞的前体。脾脏抑制因子活性与自然杀伤因子（NK）活性无明显关系；这些活动的动力学在新生儿脾脏似乎是负相关的。然而，抑制细胞与NK细胞相似，因为它们都是在没有抗原挑战的情况下发现的，缺乏抗原特异性，并且具有零表面表型。因此，我们将前者称为自然抑制细胞（NS）。
We studied the surface markers of suppressor cells of the mixed leukocyte reaction (MLR) that are transiently present in the spleens of neonatal mice after birth and of adult mice after total lymphoid irradiation (TLI). Approximately 80% of the mononuclear cells in the spleen, within the first few days after birth or after TLI, express neither the Thy-1 antigen nor surface immunoglobulin (Ig). After 30 days, less than 20% of mononuclear cells bear this null phenotype. With the use of the panning technique, we showed that the suppressors of the MLR are confined to the null cell population. The null suppressor cells are not macrophages because they did not carry macrophage markers identified by the monoclonal antibodies anti-MAC-1 and F4/80. In addition, the suppressor cells did not stain for nonspecific esterase and did not adhere firmly to plastic or glass. Spleen cells from TLI-treated mice maintained their suppressive capacity after culture in vitro for 6 to 8 wk. The cultured suppressor cells did not develop mature T cell, B cell, or macrophage markers during this time interval. Thus, the suppressor cells did not appear to be precursors of the latter cells. There was no clear relationship between the suppressor activity of the spleens and natural killer (NK) activity; the kinetics of these activities in newborn spleen appear to be inversely related. The suppressor cells, however, are similar to NK cells in that both are found in the absence of antigenic challenge, lack antigen specificity, and bear the null surface phenotype. Thus, we have termed the former cells natural suppressor (NS) cells.