Protective effect of tetrahydroxystilbene glucoside on 6-OHDA-induced apoptosis in PC12 cells through the ROS-NO pathway.
Protective effect of tetrahydroxystilbene glucoside on 6-OHDA-induced apoptosis in PC12 cells through the ROS-NO pathway.
复制标题
DOI：
10.1371/journal.pone.0026055
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Tao L;Li X;Zhang L;Tian J;Li X;Sun X;Li X;Jiang L;Zhang X;Chen J
氧化应激在帕金森病等神经退行性疾病的发病机制中起着重要作用。2，3，5，4 ′-四氢氧二苯乙烯-2-O-β-D-葡萄糖苷（TSG）是一种从中药何首乌中提取的有效抗氧化剂。本研究探讨了TSG对6-羟基多巴胺诱导的大鼠肾上腺嗜铬细胞瘤PC 12细胞凋亡的保护作用及其可能的机制。我们的数据表明，TSG显着逆转6-羟基多巴胺诱导的细胞活力的下降，防止6-羟基多巴胺诱导的浓缩核的变化，并以剂量依赖性的方式降低凋亡细胞的百分比。此外，TSG减缓了细胞内活性氧和一氧化氮的积累，抵消了诱导型一氧化氮合成以及神经元一氧化氮合成的过度表达，还降低了蛋白结合的3-硝基酪氨酸的水平。这些结果表明，TSG对大鼠肾上腺嗜铬细胞瘤PC 12细胞的保护作用至少部分是通过ROS-NO途径介导的。我们的研究结果表明，TSG可以有效地提供保护，防止与氧化应激相关的神经退行性疾病。
Oxidative stress plays an important role in the pathogenesis of neurodegenerative diseases, such as Parkinson's disease. The molecule, 2,3,5,4′-tetrahydr- oxystilbene-2-O-β-D-glucoside (TSG), is a potent antioxidant derived from the Chinese herb, Polygonum multiflorum Thunb. In this study, we investigated the protective effect of TSG against 6-hydroxydopamine-induced apoptosis in rat adrenal pheochromocytoma PC12 cells and the possible mechanisms. Our data demonstrated that TSG significantly reversed the 6-hydroxydopamine-induced decrease in cell viability, prevented 6-hydroxydopamine-induced changes in condensed nuclei and decreased the percentage of apoptotic cells in a dose-dependent manner. In addition, TSG slowed the accumulation of intracellular reactive oxygen species and nitric oxide, counteracted the overexpression of inducible nitric oxide syntheses as well as neuronal nitric oxide syntheses, and also reduced the level of protein-bound 3-nitrotyrosine. These results demonstrate that the protective effects of TSG on rat adrenal pheochromocytoma PC12 cells are mediated, at least in part, by the ROS-NO pathway. Our results indicate that TSG may be effective in providing protection against neurodegenerative diseases associated with oxidative stress.