mummy/cystic encodes an enzyme required for chitin and glycan synthesis, involved in trachea, embryonic cuticle and CNS development -: Analysis of its role in Drosophila tracheal morphogenesis
mummy/cystic encodes an enzyme required for chitin and glycan synthesis, involved in trachea, embryonic cuticle and CNS development -: Analysis of its role in Drosophila tracheal morphogenesis
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DOI：
10.1016/j.ydbio.2005.09.031
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发表时间：
2005-12-01
影响因子：
2.7
通讯作者：
Casanova, J
中科院分区：
文献类型：
--
作者：
Araújo, SJ;Aslam, H;Casanova, J
气管和神经系统发育是果蝇器官发生研究的两个模式系统。在两个独立的筛选中，我们发现了一个基因的三个等位基因，涉及气管、角质层和中枢神经系统的发育。在这里，我们表明这些等位基因，以及之前鉴定的囊性和木乃伊，都属于同一个互补组。这些是编码udp - n -乙酰氨基葡萄糖二磷酸化酶的基因突变体。一种负责产生udp - n -乙酰氨基葡萄糖的酶，是几丁质和聚糖生物合成的重要中间体。囊肿最初被认为是调节气管管直径所需的基因。我们描述了囊肿/ my气管表型，并通过组织学检查得出结论，my突变胚胎缺乏含有几丁质的结构，如表皮的小泡和气管管腔的带状褶皱。虽然他们的气管形态发生缺陷大多数可归因于缺乏几丁质，但与krotzkopf verkehrt （kkv）几丁质合成酶突变体相比，mmy突变体表现出更强的表型，这表明一些mmy表型。像轴突引导缺陷一样，是与几丁质无关的。我们讨论了这些新数据在果蝇气管大小控制机制中的意义。(C) 2005爱思唯尔公司版权所有。
Tracheal and nervous system development are two model systems for the study of organogenesis in Drosophila. In two independent screens, we identified three alleles of a gene involved in tracheal, cuticle and CNS development. Here, we show that these alleles, and the previously identified cystic and mummy, all belong to the same complementation group. These are mutants of a gene encoding the UDP-N-acetylglucosamine diphosphorylase.. an enzyme responsible for the production of UDP-N-acetylglucosamine, an important intermediate in chitin and glycan biosynthesis. cyst was originally singled out as a gene required for the regulation of tracheal tube diameter. We characterized the cyst/mmy tracheal phenotype and upon histological examination concluded that mmy mutant embryos lack chitin-containing structures, such as the procuticle at the epidermis and the taenidial folds in the tracheal lumen. While most of their tracheal morphogenesis defects can be attributed to the lack of chitin, when compared to krotzkopf verkehrt (kkv) chitin-synthase mutants, mmy mutants showed a stronger phenotype, suggesting that some of the mmy phenotypes.. like the axon guidance defects, are chitin-independent. We discuss the implications of these new data in the mechanism of size control in the Drosophila trachea. (C) 2005 Elsevier Inc. All rights reserved.