IMMUNOCYTOLOGIC DETECTION OF ISOLATED TUMOR-CELLS IN BONE-MARROW OF PATIENTS WITH SQUAMOUS-CELL CARCINOMAS OF THE HEAD AND NECK REGION
IMMUNOCYTOLOGIC DETECTION OF ISOLATED TUMOR-CELLS IN BONE-MARROW OF PATIENTS WITH SQUAMOUS-CELL CARCINOMAS OF THE HEAD AND NECK REGION
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DOI：
10.1016/s0901-5027(05)80490-4
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发表时间：
1995-10-01
影响因子：
2.4
通讯作者：
PANTEL, K
中科院分区：
文献类型：
--
作者：
GATH, HJ;HEISSLER, E;PANTEL, K
抗细胞角蛋白单克隆抗体检测骨髓中上皮微转移癌细胞作为预后指标的良好记录的特异性启发我们将这种方法应用于头颈部鳞状细胞癌（SSC）患者。通过对来自相应原发性肿瘤的冷冻切片进行免疫染色，证明了用于肿瘤细胞检测的广谱抗细胞角蛋白单克隆抗体（mAb）A45-B/B3的灵敏度。对31例SSC患者的分析显示，10例（32.3%）患者的A45-B/B3阳性细胞频率为1-207/1 × 10（6）单核细胞。大多数标本显示孤立的肿瘤细胞，而细胞簇中发现只有2例（6.5%）。目前的数据表明，癌细胞的血行播散比头颈部SSC患者的临床病理分期预期的更频繁。
The well-documented specificity of anticytokeratin monoclonal antibodies for detection of epithelial micrometastatic cancer cells in bone marrow as a prognostic indicator inspired us to apply this approach to patients with squamous cell carcinomas (SSC) of the head and neck region. The sensitivity of the broad-spectrum anticytokeratin monoclonal antibody (mAb) A45-B/B3 used for tumor cell-detection was demonstrated by immunostaining of cryostat sections from the respective primary tumors. Analysis of 31 patients with SSC revealed A45-B/B3-positive cells in 10 cases (32.3%) at frequencies of 1-207 per 1x10(6) mononuclear cells. Most specimens displayed isolated tumor cells, while cell clusters were found in only two cases (6.5%). The present data suggest that hematogenous dissemination of cancer cells is more frequent than expected from clinicopathologic staging of patients with SSC of the head and neck region.