An oligofluorene truxene based distributed feedback laser for biosensing applications.
An oligofluorene truxene based distributed feedback laser for biosensing applications.
复制标题
DOI：
10.1016/j.bios.2013.11.054
复制
发表时间：
2014-04
影响因子：
12.6
通讯作者：
Anne-Marie Haughey;B. Guilhabert;A. Kanibolotsky;P. Skabara;M. Dawson;G. Burley;N. Laurand
中科院分区：
文献类型：
--
作者：
Anne-Marie Haughey;B. Guilhabert;A. Kanibolotsky;P. Skabara;M. Dawson;G. Burley;N. Laurand
报道了基于分布反馈激光器的全有机低聚芴truxene用于检测特定蛋白质-小分子相互作用的第一个例子。使用我们的生物素标记的生物传感器平台，可检测到低至1 μ g mL− 1的抗生物素蛋白。当用脱硫生物素代替生物素时，这种相互作用是选择性的和可逆的。抗生物素蛋白检测不受高达50，000 μ g mL− 1的牛血清白蛋白干扰。我们的生物传感器提供了一个新的检测平台，既高度敏感，模块化和潜在的可重复使用。
The first example of an all-organic oligofluorene truxene based distributed feedback laser for the detection of a specific protein–small molecule interaction is reported. The protein avidin was detected down to 1 μ g mL− 1 using our biotin-labelled biosensor platform. This interaction was both selective and reversible when biotin was replaced with desthiobiotin. Avidin detection was not perturbed by Bovine Serum Albumin up to 50, 000 μ g mL− 1. Our biosensor offers a new detection platform that is both highly sensitive, modular and potentially re-usable.