Label-free reading of microarray-based immunoassays with surface plasmon resonance imaging
Label-free reading of microarray-based immunoassays with surface plasmon resonance imaging
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DOI：
10.1021/ac049318q
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发表时间：
2004-12-15
影响因子：
7.4
通讯作者：
McDermott, MT
中科院分区：
文献类型：
--
作者：
Kanda, V;Kariuki, JK;McDermott, MT
提出了一种在金表面形成蛋白质抗原图案的简单方法，用于表面等离子共振 (SPR) 成像实验。由聚（二甲基硅氧烷）制成的微流体装置用于使各种流体在金基板上的空间限定通道中流动。该技术用于对金的表面化学进行图案化，并将抗原从溶液吸附到修饰的基质上。用互补抗体探测所得的抗原阵列，以证明抗体捕获实验图案化的有效性。 SPR 成像用于帮助优化阵列制造并观察未标记抗体与这些微阵列的相互作用。 Ibis 的工作提出了一种制造具有受控抗原表面密度的微阵列的方法。 SPR 成像以无标记的形式提供抗体结合的定量和定性评估。
A simple method is presented for patterning of protein antigens at a gold surface for use in surface plasmon resonance (SPR) imaging experiments. Microfluidic devices fabricated from poly(dimethylsiloxane) were used to flow various fluids over a gold substrate in spatially defined channels. This technique was used to pattern the surface chemistry of the gold as well as to adsorb antigens from solution to the modified substrates. The resulting antigen arrays were probed with complementary antibodies in order to demonstrate the effectiveness of the patterning for antibody capture experiments. SPR imaging was used to aid in the optimization of array fabrication and to observe the interactions of unlabeled antibodies with these microarrays. Ibis work presents a means of fabricating microarrays with controlled surface density of antigens. SPR imaging provides both quantitative and qualitative evaluation of antibody binding in a label free format.