Electron microscopic tomography of rat-liver mitochondria and their interactions with the endoplasmic reticulum
Electron microscopic tomography of rat-liver mitochondria and their interactions with the endoplasmic reticulum
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DOI：
10.1002/biof.5520080309
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发表时间：
1998-01-01
期刊：
影响因子：
6
通讯作者：
Marko, M
中科院分区：
文献类型：
--
作者：
Mannella, CA;Buttle, K;Marko, M
电子显微镜断层扫描可以提供有关亚细胞结构的完整三维信息。应用于线粒体，它已经表明，传统的教科书模型这种细胞器是不正确的。内膜的内折(称为脊)由狭窄的管状区相互连接，并与外部连接，这表明离子、代谢物和蛋白质的内部扩散可能受到限制。线粒体的原位断层重建表明，这些细胞器可能与内质网堆叠在一起，形成可能参与[Ca++]调节的延伸结构。
Electron microscopic tomography can provide a complete three-dimensional range of information about subcellular structures. Applied to mitochondria, it has shown that the conventional textbook model of this organelle is incorrect. The infoldings of the inner membrane (called cristae) are connected by narrow tubular regions to each other and to the outside, suggesting that internal diffusion of ions, metabolites and proteins may be restricted. Tomographic reconstruction of mitochondria in situ indicates these organelles may occur in clusters with stacks of endoplasmic reticulum, forming extended structures that may be involved in [Ca++] regulation.