ACTIVATION OF ARACHIDONIC-ACID METABOLISM IN MOUSE MACROPHAGES BY BACTERIAL AMPHIPHILES
ACTIVATION OF ARACHIDONIC-ACID METABOLISM IN MOUSE MACROPHAGES BY BACTERIAL AMPHIPHILES
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DOI：
10.1002/jlb.56.6.723
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发表时间：
1994-12-01
影响因子：
5.5
通讯作者：
GUSTAFSON, GL
中科院分区：
文献类型：
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作者：
CARD, GL;JASUJA, RR;GUSTAFSON, GL
比较了4种革兰氏阳性菌的脂磷壁酸（LTA）与不同脂多糖（LPS）制剂对小鼠腹腔巨噬细胞花生四烯酸代谢活化的相对活性。在用[H-3]-花生四烯酸标记的培养物中测定总类花生酸。前列腺素E（2）（PGE（2））和白三烯C-4。（LTC（4））通过EIA分析测定，不同制剂的相对效价为：来自流产沙门氏菌的光滑LPS大于或等于来自沙门氏菌的Re-LPS（R-595）大于或等于来自化脓链球菌的LTA接近粪链球菌接近金黄色葡萄球菌大于或等于来自Re-LPS的单磷酰脂质A> >来自枯草芽孢杆菌的LTA。对于所有测试的两亲物，星形孢菌素抑制类花生酸释放的激活。pyogenes、S. faecalis和S.金黄色葡萄球菌，在存在或不存在吲哚美辛的情况下，在随后用LPS攻击时使细胞对类花生酸释放脱敏。脱敏的细胞保持对佛波酯佛波醇肉豆蔻酸酯乙酸酯的应答。来自革兰氏阴性菌的LPS具有免疫刺激和内毒素活性，这部分是由于从活化的巨噬细胞释放类二十烷酸和其他介质。LPS和LTA激活细胞模式的相似性表明，脂磷壁酸可能有助于革兰氏阳性菌的致病性。
The relative activities of lipoteichoic acid (LTA) from four Gram-positive bacteria were compared to different lipopolysaccharide (LPS) preparations for activation of arachidonic acid metabolism in mouse peritoneal macrophages. Total eicosanoid was determined in cultures labeled with [H-3]-arachidonic acid. Prostaglandin E(2) (PGE(2)) and leukotriene C-4. (LTC(4)) were determined by EIA analysis, The relative potencies of the different preparations were: smooth LPS from Salmonella abortus greater than or equal to Re-LPS from Salmonella minnesota (R-595) greater than or equal to LTA from Streptococcus pyogenes approximate to Streptococcus faecalis approximate to Staphylococcus aureus greater than or equal to monophosphoryl lipid A derived from the Re-LPS > > LTA from Bacillus subtilis. Activation of eicosanoid release was inhibited by staurosporin for all of the amphiphiles tested, Treatment of the macrophage cultures with LTA from S. pyogenes, S. faecalis, and S. aureus, either in the presence or absence of indomethacin, desensitized the cells to eicosanoid release on subsequent challenge with LPS, The desensitized cells remained responsive to the phorbol ester phorbol myristate acetate. LPS from Gram-negative bacteria has immunostimulatory and endotoxic activities which result, in part, from the release of eicosanoids and other mediators from activated macrophages. The similarities in the patterns of cell activation by LPS and LTA suggest that lipoteichoic acids might contribute to the pathogenicities of Gram-positive bacteria.